NUDCD2 Antibody

Definition and Target

NUDCD2 (NudC domain-containing 2) is a human protein encoded by the NUDCD2 gene (NCBI Gene ID: 134492). It functions as an HSP90 cochaperone, stabilizing proteins like LIS1 in the dynein pathway and regulating sister chromatid cohesion during mitosis . The NUDCD2 antibody is a polyclonal or recombinant antibody designed to bind specifically to this protein for experimental detection.

Table 1: Antibody Variants and Properties

Validation Data

The 21205-1-AP antibody has been validated across multiple applications:

Table 2: Application-Specific Validation

Research Applications

• Mitotic Regulation: NUDCD2 ensures sister chromatid cohesion during mitosis by stabilizing LIS1, a dynein regulator, via HSP90 .

• Ciliogenesis: NUDCD2 supports the formation of cilia, critical for cellular signaling .

• Disease Associations: Dysregulation of NUDCD2 may contribute to cancer progression, as evidenced by its detection in breast cancer tissues .

Key Research Findings

• Structural Role: NUDCD2 stabilizes LIS1, enabling proper dynein motor complex assembly and mitotic spindle orientation .

• HSP90 Interaction: Acts as an HSP90 cochaperone, facilitating protein folding and complex assembly .

• Clinical Relevance: Detected in breast cancer biopsies, suggesting potential as a diagnostic or therapeutic target .

Protocol Considerations

• Antigen Retrieval: For IHC, TE buffer (pH 9.0) or citrate buffer (pH 6.0) is recommended .

• Storage Stability: Both antibodies retain functionality for ≥1 year at -20°C .

Background and Significance

The NUDCD2 gene resides on chromosome 5 (GRCh38.p14) and produces an 18 kDa protein. Its interplay with HSP90 and dynein underscores its importance in cell division and structural integrity, making it a focal point for cancer and developmental biology research .