NUDT1 Human
Description
DNA/RNA Sanitization
NUDT1 prevents the misincorporation of oxidized nucleotides during replication and transcription, thereby mitigating oxidative DNA damage (ODD) . This is critical in:
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Cancer cells: High NUDT1 expression counteracts MYC-driven reactive oxygen species (ROS) production, enabling survival under oxidative stress .
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Aging: Overexpression in mice reduces 8-oxoguanine levels in DNA, correlating with a 16% lifespan extension .
Regulation and Activity
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MYC-PLK1 Axis: MYC overexpression induces PLK1-mediated phosphorylation of NUDT1, enhancing its enzymatic activity without altering protein levels .
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FOXM1 Dependency: In pulmonary arterial hypertension (PAH), FOXM1 upregulates NUDT1 to protect against oxidative damage .
Cancer
NUDT1 is overexpressed in aggressive cancers, including:
Aging and Neurodegeneration
NUDT1-null mice exhibit elevated 8-oxo-dGTP and accelerated aging, while overexpression delays oxidative damage accumulation .
Inhibitors and Mechanisms
Controversies: Early inhibitors like TH588 showed promise, but later studies (e.g., AstraZeneca, MD Anderson) reported limited efficacy, questioning NUDT1’s validity as a target .
Prognostic and Diagnostic Value
NUDT1 serves as a biomarker in:
Product Specs
MGSSHHHHHH SSGLVPRGSH MGASRLYTLV LVLQPQRVLL GMKKRGFGAG RWNGFGGKVQ EGETIEDGAR RELQEESGLT VDALHKVGQI VFEFVGEPEL MDVHVFCTDS IQGTPVESDE MRPCWFQLDQ IPFKDMWPDD SYWFPLLLQK KKFHGYFKFQ GQDTILDYTL REVDTV.
Product Science Overview
NUDT1 hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP and 8-oxo-dATP, into their corresponding monophosphates. This activity prevents the incorporation of these oxidized nucleotides into DNA, which could otherwise lead to mutations and genomic instability . The enzyme is predominantly found in the cytoplasm, with some presence in the mitochondria, indicating its role in sanitizing nucleotide pools for both nuclear and mitochondrial genomes .
The human recombinant form of NUDT1 is typically produced in Escherichia coli and is a single, non-glycosylated polypeptide chain containing 176 amino acids. It is often fused with a 20 amino acid His tag at the N-terminus to facilitate purification . The enzyme has a molecular mass of approximately 20.1 kDa .
The recombinant NUDT1 is purified using proprietary chromatographic techniques to achieve a purity greater than 95%, as determined by SDS-PAGE . The enzyme is formulated in a solution containing Tris-HCl buffer, glycerol, dithiothreitol (DTT), and sodium chloride (NaCl) to ensure stability and activity .
