NUMBL Antibody
Description
Target Protein Overview
NUMBL is a 65-70 kDa cytoplasmic protein encoded by the NUMBL gene (NCBI Gene ID: 9253) that shares structural and functional homology with Drosophila Numb . Key biological functions include:
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Regulation of clathrin-mediated endocytosis
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Maintenance of neural progenitor cells during embryonic neurogenesis
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Survival of subventricular zone neuroblasts in postnatal brains
Antibody Characteristics
Commercial NUMBL antibodies demonstrate distinct technical profiles:
All listed antibodies are IgG isotype and require antigen retrieval methods for optimal performance in formalin-fixed tissues .
Western Blot Performance
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10111-1-AP detects 65-70 kDa bands in mouse brain, spleen, lung, liver, and HeLa cell lysates
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ABIN658002 shows specific reactivity in human tissue lysates
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HPA058251 demonstrates 65 kDa band in human cell lines with enhanced validation
Immunohistochemical Localization
Key Experimental Uses
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Neural Development Studies: Used to demonstrate NUMBL's role in radial glial cell maintenance through Notch signaling modulation
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Cancer Research: Applied in detecting NUMBL expression patterns in lung cancer metastasis models
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Cellular Imaging: Enables subcellular localization analysis in PC-12 and HepG2 cell lines
Protocol Recommendations
| Application | Dilution Range | Antigen Retrieval |
|---|---|---|
| Western Blot | 1:1000-1:4000 | Not required |
| IHC | 1:50-1:500 | TE buffer (pH 9.0) or citrate buffer (pH 6.0) |
| Immunofluorescence | 1:50-1:500 | Methanol fixation |
Quality Control Metrics
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Batch Consistency: Protein A affinity purification with peptide verification ensures <10% lot-to-lot variability
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Specificity Confirmation:
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Stability: Maintains activity for 12 months at -20°C in 50% glycerol buffer
Research Findings
Recent studies using these antibodies have revealed:
Product Specs
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- NUMBL can function as an independent tumor suppressor, inhibiting the Notch pathway and regulating the cancer stem cell pool. PMID: 27613838
- let-7c inhibits Notch and progression markers but upregulates NUMBL in pancreatic cancer treated with quercetin. PMID: 27521217
- NUMBL may be involved in the inhibition of growth, proliferation, and invasion of 95-D lung cancer cells. PMID: 23681800
- NUMBL-Klf4 signaling is crucial for maintaining multiple nodes of metastatic progression, including the persistence of cancer-initiating cells. PMID: 23440423
- Research indicates that NUMBL regulates glioma cell migration and invasion by blocking TRAF5-induced activation of NF-KappaB. PMID: 22593207
- NUMBL interacts with TRAF6 and promotes its degradation in vivo, leading to the inhibition of the NF-kappaB signaling pathway. PMID: 20079715
- Both gene sequence alterations and amplifications of LNX1 and NUMBL are present in a subset of human gliomas. PMID: 18940473
