NUP160 Antibody
Description
Overview of NUP160 Antibody
The NUP160 Antibody (STJ94576) is a rabbit polyclonal antibody specifically targeting the nuclear pore complex protein NUP160. This antibody is designed for research applications, including Western Blot (WB) and Enzyme-Linked Immunosorbent Assay (ELISA), and is strictly limited to non-diagnostic or therapeutic uses .
Functionality of NUP160 in Cellular Processes
NUP160 is a critical component of the nucleoporin complex, which regulates nucleocytoplasmic transport . Research highlights its role in:
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mRNA Export: NUP160 interacts with Nup98 and Nup153 to facilitate mRNA export .
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Immune Function: Studies link NUP160 complex members to antigen presentation and viral susceptibility .
3.1. Western Blot (WB)
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Dilution Range: 1:500–1:2000.
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Detection: Identifies endogenous NUP160 protein levels in human/mouse lysates .
3.2. ELISA
4.1. Role in mRNA Export
NUP160, along with Nup133, forms a subcomplex that tethers Nup98 and Nup153 to nuclear pores, enabling mRNA export .
4.2. Immune and Viral Pathways
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Nup160 complex members are implicated in antigen presentation and viral infection susceptibility .
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Viruses like HSV1 and adenoviruses exploit interactions with nucleoporins (e.g., NUP214) for nuclear entry .
Technical Considerations
Product Specs
Target Background
- The Nup107-160 complex and its partner Elys dynamically colocalize with Nup98 and CRM1 into endogenous GLFG bodies present in specific HeLa sublines. PMID: 25904327
- Transcriptome sequencing of patient-derived angiosarcoma cells revealed a novel fusion gene, NUP160-SLC43A3, expressed in 9 out of 25 human angiosarcoma specimens examined. PMID: 26527604
- The SUMO-specific isopeptidase SENP2 dynamically associates with nuclear pore complexes through interactions with karyopherins and the Nup107-160 nucleoporin subcomplex. PMID: 22031293
- Interactions between Nup160 and Pom121 are predicted to facilitate the formation of the nuclear pore and the anchoring of the nuclear pore complexes to the pore membrane. PMID: 20974814
- A primary function of the Nup107 complex during mitosis is to ensure the proper localization of the chromosome passenger complex at the centromere. PMID: 19864462
- Data indicate that the Nup107-160 complex promotes spindle assembly through Ran-GTP-regulated nucleation of microtubules by gamma-TuRC at kinetochores. This reveals a connection between nucleoporins and the microtubule cytoskeleton. PMID: 20081840
- Structure-guided sequence comparison of Nup160 and Saccharomyces cerevisiae Nup120 strongly suggests that both proteins adopt the same unique fold despite a low sequence identity of 10%. PMID: 19576787
- The Nup107-160 complex exhibits dynamic behavior during mitosis and plays a distinct role in promoting spindle assembly compared to its functions at interphase nuclear pores. PMID: 16807356
- Phosphorylation does not affect interactions within the Nup107-160 subcomplex but regulates its association with the NPC and other proteins. PMID: 17360435
- These findings provide the first molecular insights into the function of the human Nup107-160 complex at kinetochores. PMID: 17363900
