NUP35 Antibody

NUP35 Antibody Overview

NUP35 antibodies are immunochemical reagents targeting the NUP35 protein, encoded by the NUP35 gene (Entrez Gene ID: 129401; UniProt ID: Q8NFH5) . These antibodies enable the detection, localization, and functional analysis of NUP35, which plays roles in mRNA export, NPC assembly, and cellular homeostasis .

mRNA Export Regulation

NUP35 antibodies have been critical in identifying the protein’s role in cardiomyocyte pH regulation. Knockdown studies using siRNA revealed that NUP35 binds the 5′-UTR (−412 to −213 nt) of nhe1 mRNA, facilitating its nuclear export. Overexpression of NUP35 mutants (e.g., GFP-Nup35ΔN-terminus) disrupted mRNA transport, linking NUP35 to intracellular acidification in ischemic conditions .

Disease Modeling

In murine models, a missense mutation (F192L) in Nup35 caused degenerative colonic smooth muscle myopathy, mimicking human chronic intestinal pseudo-obstruction (CIPO). Immunohistochemistry with NUP35 antibodies revealed muscle cell loss, fibrosis, and macrophage infiltration in affected tissues .

Protein Localization

NUP35 antibodies are used in the Human Protein Atlas to map subcellular localization, confirming NUP35’s presence at the nuclear rim and its association with NPCs .

Key Research Findings

Functional Insights

• Domain Specificity: The N-terminal domain of NUP35 is essential for mRNA binding, while the RRM domain stabilizes interactions .

• Disease Link: NUP35 dysfunction correlates with NPC degradation in neurodegenerative diseases and age-related transport defects .

• Conservation: NUP35 homologs (e.g., yeast Nup53) share structural roles in NPC assembly, underscoring evolutionary importance .

Limitations and Considerations

• Species Specificity: Most antibodies target human NUP35; cross-reactivity with rodent isoforms varies .

• Functional Redundancy: Partial compensation by other nucleoporins may obscure phenotypes in knockout models .