NXPH4 Antibody

Definition and Target Specificity

The NXPH4 antibody (e.g., Thermo Fisher PA5-101711) is a polyclonal antibody raised against recombinant fragments of human NXPH4. It detects endogenous levels of total NXPH4, a 308-amino-acid secreted protein belonging to the neurexophilin family . NXPH4 contains five conserved domains, including a glycosylated central region and cysteine-rich C-terminal domain, which facilitate its role as a signaling molecule binding to α-neurexins . The antibody is validated for Western blot (WB), immunohistochemistry (IHC), and ELISA, with reactivity confirmed in human, mouse, and rat samples .

Applications in Cancer and Neurological Research

NXPH4 antibody has been pivotal in elucidating the protein’s dual role in synaptic function and oncogenesis:

• Cancer Biomarker Studies:

  • Western blot analyses using NXPH4 antibody revealed upregulated expression in colon cancer (HT-29, HCT116), liver hepatocellular carcinoma (HCC), and other malignancies .

  • In HCC, high NXPH4 levels correlate with poor prognosis, immune cell infiltration (CD8+ T cells, macrophages), and resistance to gemcitabine .

• Neurological Research:

  • While endogenous NXPH4 detection in brain circuits remains challenging due to antibody limitations, knock-in mouse models (e.g., Nxph4-βgeo) highlight its role in motor coordination and synaptic adhesion .