odr-10 Antibody
Description
Epitope Tag Antibodies in Odr-10 Research
| Tag | Antibody | Application | Source |
|---|---|---|---|
| GFP | Anti-GFP | Localization in AWA sensory cilia | |
| Myc | Anti-Myc | Surface expression in HEK293 cells | |
| V5 | Anti-V5 | Western blot confirmation of purification |
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GFP-tagged Odr-10: Used to visualize localization in AWA neurons, confirming its presence in sensory cilia .
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Myc-tagged Odr-10: Detected in transiently transfected HEK293 cells to study diacetyl-induced Ca²⁺ signaling .
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V5-tagged Odr-10: Validated recombinant protein expression in E. coli and reconstitution in detergent micelles .
Functional Characterization in Heterologous Systems
In HEK293 cells, Myc-ODR-10 expression was confirmed via anti-Myc antibodies, enabling studies on diacetyl-induced intracellular Ca²⁺ elevation. Key findings include:
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Specificity: No response to structurally similar compounds like butanone .
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Cross-reactivity: Pyruvate and citrate (bacterial metabolites) also activated Odr-10, suggesting a broader ligand range .
| Ligand | Response | EC₅₀ | Source |
|---|---|---|---|
| Diacetyl | Ca²⁺ elevation | 3.2 μM | |
| Pyruvate | Ca²⁺ elevation | 3.2 μM | |
| Citrate | Ca²⁺ elevation | N/A |
Limitations and Challenges
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No Native Antibodies: No reports exist of antibodies targeting untagged Odr-10, limiting studies to engineered systems.
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Tag Dependency: Detection relies on tag expression, which may alter receptor function or localization .
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Human Olfactory Receptor Antibodies: Commercial antibodies like anti-OR10R2 or anti-OR10X1 target distinct human receptors and are unrelated to C. elegans Odr-10.
Product Specs
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
