Omentin Antibody
Description
Introduction to Omentin Antibody
Omentin antibodies are specialized immunoglobulins designed to bind and detect omentin, a protein secreted by visceral adipose tissue and other cell types. Omentin is a 313-amino-acid adipokine with two isoforms (omentin-1 and omentin-2) that share 83% sequence identity . These antibodies enable researchers to study omentin’s roles in insulin sensitivity, inflammation, atherosclerosis, and metabolic disorders.
Types of Omentin Antibodies
Omentin antibodies are categorized by their production method and specificity:
Note: Polyclonal antibodies (e.g., AF4254) are often used for immunohistochemistry, while monoclonals (e.g., MAB4254) are preferred for Western blotting and ELISA .
Applications in Research
Omentin antibodies are critical tools in diverse experimental workflows:
Western Blotting
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Detects omentin-1 in plasma and tissue lysates, with a predicted molecular weight (MW) of 35–40 kDa (glycosylated forms may appear as dimers or trimers) .
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Example: Recombinant human omentin-1 (Thr19-Ser298) is resolved at ~35 kDa under reducing conditions .
Immunohistochemistry (IHC)
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Localizes omentin in Paneth cells (mouse intestine), U937 lymphoma cells, and vascular smooth muscle cells .
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Protocol: Frozen sections stained with AF4254 (10 µg/mL) and NorthernLights™ 557-conjugated secondary antibodies .
ELISA Development
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MAB4254 serves as a capture antibody for sandwich ELISA, paired with biotinylated detection antibodies .
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Sensitivity: Detects recombinant omentin-1 at concentrations as low as 0.5 ng/mL .
Metabolic and Cardiovascular Insights
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Obesity and Insulin Resistance
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Atherosclerosis
Cellular Mechanisms
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Anti-inflammatory Effects: Omentin-1 promotes M2 macrophage polarization, confirmed via IHC in Apoe−/− mice .
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Vascular Protection: Antibodies showed omentin-1 suppresses angiotensin II-induced smooth muscle cell migration and PDGF-BB-induced proliferation .
Challenges and Considerations
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Cross-Reactivity: Polyclonal antibodies may detect both omentin-1 and omentin-2, necessitating isoform-specific validation .
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Storage: Antibodies require -20°C storage (avoid repeated freeze-thaw cycles). Azide-free formulations are recommended for long-term storage .
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Species Specificity: Human antibodies (e.g., AF4254) do not cross-react with mouse omentin, which lacks glycosylation .
Product Specs
Q&A
Here’s a structured collection of FAQs tailored for academic researchers working with Omentin antibodies, based on technical and methodological insights from peer-reviewed studies:
Advanced Research Questions
How to resolve contradictions in Omentin expression data across studies?
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Analytical framework:
What strategies address cross-reactivity with Intelectin-2 or other homologs?
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Approaches:
How to optimize Omentin antibody use in multiplex assays (e.g., flow cytometry)?
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Technical adjustments:
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Titrate antibodies to minimize spectral overlap (e.g., PE-conjugated anti-Omentin at 1:50 dilution) .
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Pre-block Fc receptors with CD16/32 antibodies to reduce nonspecific binding in immune cells .
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Combine with intracellular staining protocols for macrophage studies (e.g., THP-1-derived macrophages) .
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Can Omentin antibodies elucidate mechanistic pathways in metabolic diseases?
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Case study:
Methodological Best Practices
Product Science Overview
Omentin, also known as omentin-1, is a novel adipokine predominantly expressed in visceral adipose tissue (VAT). It plays a crucial role in various metabolic processes and has been linked to obesity, insulin resistance, and cardiovascular diseases . Omentin-1 is known for its anti-inflammatory and insulin-sensitizing properties, making it a significant molecule in metabolic research .
Omentin-1 enhances insulin-mediated glucose uptake in adipocytes, contributing to improved insulin sensitivity . It also exhibits anti-inflammatory effects by inhibiting the expression of pro-inflammatory cytokines such as TNF-α and reducing oxidative stress . Additionally, omentin-1 promotes vasodilation and revascularization, which are beneficial for cardiovascular health .
Research has shown that omentin-1 levels are decreased in individuals with obesity, insulin resistance, and type 2 diabetes . Lower levels of omentin-1 have also been observed in patients with polycystic ovary syndrome (PCOS) and metabolic dysfunction-associated steatotic liver disease (MASLD) . These findings suggest that omentin-1 plays a protective role against metabolic and cardiovascular diseases.
The mouse anti-human omentin antibody is a monoclonal antibody specifically designed to target and bind to human omentin-1. This antibody is commonly used in research to study the expression and regulation of omentin-1 in various tissues and disease states . It is utilized in techniques such as Western blotting, immunohistochemistry, and enzyme-linked immunosorbent assays (ELISA) to detect and quantify omentin-1 levels .
The mouse anti-human omentin antibody has been instrumental in advancing our understanding of omentin-1’s role in metabolic diseases. For instance, studies using this antibody have demonstrated that insulin and glucose can downregulate omentin-1 expression in adipose tissue . Additionally, research has shown that omentin-1 supplementation can reduce inflammation and oxidative stress in vitro and ex vivo models .
