omp Antibody, FITC conjugated

Definition and Overview

"Omp Antibody, FITC conjugated" refers to a fluorescently labeled immunoglobulin designed to detect specific target proteins, either olfactory marker protein (OMP) in mammalian systems or bacterial outer membrane proteins (OMPs). The fluorescein isothiocyanate (FITC) conjugation enables visualization of antibody-antigen interactions in techniques like immunofluorescence, flow cytometry, and immunohistochemistry .

Mammalian Olfactory Marker Protein (OMP)

• Function: OMP is a cytoplasmic protein in mature olfactory neurons, modulating odor detection and signal transduction .

• Antibody Specifications:

  Property	Details
  Host Species	Mouse (monoclonal, clone B-6) or Rabbit (polyclonal)
  Reactivity	Mouse, Rat, Human
  Applications	WB, IP, IF, IHC, ELISA
  Conjugate Options	FITC, HRP, PE, Alexa Fluor® variants
  Concentration	200 µg/mL (Santa Cruz Biotechnology)

Bacterial Outer Membrane Proteins (OMPs)

• Example Target: Rickettsia japonica 17 kDa surface antigen (UniProt: Q52764) .

• Antibody Specifications:

  Property	Details
  Host Species	Rabbit (polyclonal)
  Reactivity	Rickettsia spp.
  Immunogen	Recombinant 17 kDa OMP (20-159 AA)
  Applications	IF, IHC, Flow Cytometry

Immunofluorescence (IF) and Flow Cytometry

• Protocol: FITC-conjugated antibodies are diluted 1:500 in PBS/10% fetal bovine serum (FBS) for cell staining, followed by fluorescence microscopy or flow cytometry .

• Key Findings:

  • Anti-OMP FITC antibodies enabled quantification of CD3+/CD4+/CD8+ T-cell subsets in murine middle ear mucosa .

  • In Acinetobacter baumannii studies, FITC-labeled anti-OmpA antibodies enhanced macrophage-mediated opsonophagocytosis .

Western Blotting (WB)

• Usage: Detects OMP in olfactory neurons or bacterial lysates at concentrations as low as 2 µg/mL .

Sensitivity and Specificity

• Affinity: Anti-OmpA monoclonal antibodies demonstrated high affinity (1.94 × 10⁻⁹ M) in ELISA .

• Cross-Reactivity: Anti-Rickettsia OMP antibodies show minimal non-specific binding in bacterial lysates .

Recent Research Findings

• Immune Modulation: Anti-PD-L1 antibodies combined with OMP-FITC increased IgA/IgG antibody-producing cells in murine models, enhancing bacterial clearance in otitis media .

• Therapeutic Potential: Anti-OmpA FITC antibodies improved macrophage bactericidal activity against multidrug-resistant A. baumannii .

Challenges and Considerations

• Labeling Efficiency: Over-conjugation with FITC reduces antigen-binding affinity and increases non-specific staining .

• Photobleaching: FITC’s fluorescence intensity diminishes under prolonged illumination, necessitating rapid imaging .