ONECUT1 Antibody
Description
Applications in Research
ONECUT1 antibodies are widely used to study:
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Pancreatic Development: ONECUT1 regulates pancreatic progenitor differentiation by priming enhancers of genes like NKX6.1, NEUROG3, and PDX1, which are essential for β-cell maturation .
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Diabetes Mechanisms: Pathogenic ONECUT1 mutations impair β-cell development and are linked to neonatal diabetes and type 2 diabetes (T2D) .
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Liver Function: ONECUT1 modulates liver-expressed genes, including those involved in glucose homeostasis and drug metabolism .
Validation Data
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Western Blot: Anti-ONECUT1 antibodies detect bands at 51–60 kDa, consistent with its theoretical molecular weight (~51 kDa). For example, PA2214 identifies ONECUT1 in HepG2 (human), RH35 (rat), and mouse liver lysates .
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Immunocytochemistry (ICC): AF6277 localizes ONECUT1 to nuclei and cytoplasm in HepG2 cells, confirming its role as a nuclear transcription factor .
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Immunohistochemistry (IHC): Proteintech’s 25137-1-AP shows strong staining in human liver cancer tissues, supporting ONECUT1’s oncogenic associations .
ONECUT1 in Pancreatic and Endocrine Development
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Binds to enhancers of FOXA2, PROX1, and SOX9 during pancreatic progenitor differentiation, facilitating endocrine specification .
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Cooperates with PDX1 and GATA6 to activate β-cell-specific programs, with disruptions causing pancreatic hypoplasia .
Disease Associations
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Rare ONECUT1 missense variants (e.g., p.P75A) are implicated in T2D susceptibility .
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Biallelic mutations cause neonatal syndromic diabetes, while monoallelic variants contribute to MODY-like phenotypes .
Limitations and Considerations
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Cross-Reactivity: Some antibodies (e.g., PA2214) show no cross-reactivity with non-target proteins, while others (e.g., 25137-1-AP) may require species-specific validation .
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Cell-Type Specificity: Bulk sequencing data may obscure heterogeneous cell populations, necessitating single-cell analyses for precise mechanistic insights .
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
Function and References:
- Reduced HNF6 expression is associated with metastasis of cholangiocarcinoma. PMID: 26825606
- HNF6 is a novel host factor that can restrict hepatitis B virus replication through both transcriptional and posttranscriptional mechanisms. PMID: 25653429
- The negative correlation between HNF6 expression and pancreatic cancer progression suggests that HNF6 maintains pancreatic epithelial homeostasis, and its loss contributes to the progression from intraepithelial neoplasms to ductal adenocarcinoma. PMID: 24638272
- HNF6 plays a significant role in the gene regulation of CYP3A4 during development from the fetal period to the postnatal period. PMID: 23291635
- HNF6 inhibits EMT, cell migration, and invasive growth through a mechanism involving the transcriptional activation of p53. PMID: 24022481
- Mutations at the -5/-6 site of the F9 promotor account for the majority of hemophilia B Leyden cases and disrupt the binding of ONECUT1 and ONECUT2. PMID: 23472758
- HNF6 and Sox9 are new biomarkers of pancreatic acinar-to-ductal metaplasia and constitute candidate targets for preventive treatment in cases when acinar-to-ductal metaplasia may lead to cancer. PMID: 22271799
- Hepatocyte nuclear factor 6 (HNF6) is one of the liver-enriched transcription factors, it utilizes the bipartite onecut-homeodomain sequence to localize to the nuclear compartment and bind specific DNA sequences of numerous target gene promoters. PMID: 21893194
- HNF6 has a role as a coactivator protein to potentiate the transcriptional activity of another liver factor, FoxA2. PMID: 12509444
- These findings define a precursor cellular stage of the embryonic pancreas and place Hnf1beta in a genetic hierarchy that regulates the generation of pancreatic endocrine cells. PMID: 14570708
- Researchers systematically identified the genes occupied by the transcriptional regulators HNF1alpha, HNF4alpha, and HNF6, together with RNA polymerase II in human liver and pancreatic islets. PMID: 14988562
- HNF6 protein stability is controlled by CBP acetylation. PMID: 15304484
- A comparison of gene expression patterns induced by hepatic nuclear factors, HNF6, HNF4alpha and HNF1beta, in a pancreatic beta-cell line. PMID: 15520459
- Results suggest that HNF-6 is a novel target of small heterodimer partner in the regulation of gluconeogenesis. PMID: 18459945
