OR1D2 Antibody
Description
What is OR1D2 Antibody?
OR1D2 antibodies are polyclonal or monoclonal reagents that bind specifically to the olfactory receptor 1D2 (OR1D2), a G-protein-coupled receptor (GPCR) encoded by the OR1D2 gene. These antibodies enable researchers to study the receptor's expression, localization, and function in various tissues .
Key Characteristics:
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Target Protein: OR1D2 (UniProt ID: P34982), a 35 kDa transmembrane protein .
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Immunogen Regions: Commonly target epitopes within amino acid residues 201–250 or 159–196 of the OR1D2 protein .
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Applications: Validated for Western blot (WB), ELISA, immunofluorescence (IF), and immunohistochemistry (IHC) .
2.1. Expression Profiling
OR1D2 is expressed in:
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Olfactory epithelium: Mediates odor detection via interaction with odorant molecules like bourgeonal .
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Spermatozoa: Localizes to the midpiece and head region upon activation, facilitating chemotaxis .
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Human airway smooth muscle cells (HASMCs): Modulates contraction and cytokine secretion .
Table 2: Potency of Selected OR1D2 Agonists
| Agonist | LogEC₅₀ | Odor Profile |
|---|---|---|
| Tetrahydromyrcenol | -6.20 | Citrus |
| Lilial | -4.38 | Floral |
| γ-Undecalactone | -4.25 | Fruity |
| 1-Octen-3-ol | -4.38 | Earthy |
Non-Olfactory Roles
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Sperm Chemotaxis: Bourgeonal activation triggers cAMP signaling, guiding sperm motility .
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Airway Physiology: In HASMCs, OR1D2 activation induces calcium influx and cytokine secretion (e.g., IL-8), influencing airway contractility and inflammation .
3.1. Antibody Performance
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Specificity: Confirmed via immunocytochemistry and Western blot in HASMCs and transfected cell lines .
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Cross-Reactivity: Detects human, rat, and mouse OR1D2 homologs .
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Storage: Stable at -20°C for ≥1 year in glycerol-containing buffers .
3.2. Experimental Workflows
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Western Blot: Membrane fractions of HASMCs show stronger OR1D2 signals than cytosolic fractions .
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Immunofluorescence: Localizes OR1D2 to the plasma membrane in sperm and airway cells .
Research Implications
OR1D2 antibodies facilitate studies on:
Product Specs
Target Background
- This preliminary study suggests a significant role for OR1D2 in both the nose and spermatozoa, potentially explaining idiopathic infertility in the study group. PMID: 23525651
- The homo-oligomerization status of the human OR1740 receptor and its involvement in receptor activation upon odorant ligand binding were investigated. PMID: 21454689
- The findings indicate that hOR17-4 functions in human sperm chemotaxis and could be a crucial component of the fertilization process. PMID: 12663925
