OR5V1 Antibody, Biotin conjugated

Overview of OR5V1 Antibody, Biotin Conjugated

OR5V1 is a G-protein-coupled olfactory receptor encoded by the OR5V1 gene (UniProt: Q9UGF6) that mediates odorant signaling in humans . The biotin-conjugated OR5V1 antibody is a rabbit-derived polyclonal IgG antibody covalently linked to biotin, enabling streptavidin-based detection systems .

Immunoassays

• ELISA: Used to quantify OR5V1 in biological samples, with recommended dilutions of 1:500–1:1000 . Biotinylated antibodies reduce background noise compared to direct HRP conjugates .

• Western Blot: Detects OR5V1 at 1:300–5,000 dilutions, though validation data are vendor-dependent .

Interference Considerations

• Biotin Cross-Reactivity: Endogenous biotin or biotin-IgM antibodies in human sera may cause false positives in streptavidin-based assays . Pre-blocking with free biotin is recommended .

• Signal Optimization: Studies show biotin-streptavidin systems enhance sensitivity by 10–100x compared to direct conjugates .

Advanced Techniques

• Proximity Biotinylation: OR5V1 antibodies conjugated to biotin have been used to map protein interaction networks at neuronal axon initial segments (AIS) .

Quality Control and Validation

• Purity: ≥95% as confirmed by Protein A/G affinity chromatography .

• Stability: Retains activity for 12 months at -20°C .

• Validation Data:

  • Western Blot: Clear bands at ~35 kDa (predicted molecular weight of OR5V1) .

  • Immunohistochemistry: Localized staining in olfactory epithelium sections .

Comparative Analysis of Biotin vs. Other Conjugates

Challenges and Limitations

• Biotin Interference: Endogenous biotin in tissues (e.g., liver, kidney) may require additional blocking steps .

• Species Reactivity: Limited to human samples; cross-reactivity with other species (e.g., mouse, rat) is untested .