OSBPL10 Antibody

Introduction

The OSBPL10 antibody is a research tool designed to detect the oxysterol-binding protein-like 10 (OSBPL10), also known as ORP10. This protein belongs to the oxysterol-binding protein (OSBP) family, which regulates cellular lipid metabolism, intracellular transport, and immune responses. The antibody has been instrumental in elucidating OSBPL10’s role in lipid-related diseases and cancer, as detailed in genetic and functional studies .

Genetic Linkage to Dyslipidemia

• High Triglyceride Trait: A 2009 study identified OSBPL10 as a candidate gene for elevated triglyceride levels in Finnish families with familial combined hyperlipidemia (FCHL) . Single-nucleotide polymorphisms (SNPs) in OSBPL10 showed suggestive linkage with extreme triglyceride levels (>90th percentile), supported by association analysis in a metabolic syndrome cohort (N = 2,138) .

• Functional Insights: RNA interference (RNAi) experiments in Huh7 hepatoma cells revealed that ORP10 suppresses hepatic lipogenesis and very-low-density lipoprotein (VLDL) production. Silencing OSBPL10 increased triglyceride synthesis and apolipoprotein B-100 secretion, suggesting its regulatory role in lipid metabolism .

Role in Pancreatic Cancer

• Immune Cell Infiltration: OSBPL10 expression correlates with immune cell infiltration in pancreatic ductal adenocarcinoma (PDAC). High OSBPL10 levels are associated with increased infiltration of CD8+ T cells, macrophages, and dendritic cells, implying a potential role in tumor immunology and immunotherapy .

Applications in Research

The OSBPL10 antibody (15491-1-AP) is validated for multiple techniques:

1. Western Blot: Detects OSBPL10 in lysates from liver or tumor tissues.

2. Immunofluorescence: Visualizes ORP10’s localization on microtubules in Huh7 cells .

3. Immunohistochemistry: Used to study OSBPL10 expression in cancer tissues .