OSBPL9 Antibody
Description
Introduction to OSBPL9 Antibody
The OSBPL9 antibody is a polyclonal rabbit IgG antibody designed to target the oxysterol-binding protein-like 9 (OSBPL9), a key regulator of cholesterol transport and Golgi apparatus function. Its primary role is to detect OSBPL9 protein expressions in human and mouse samples via Western blot (WB), immunohistochemistry (IHC), and enzyme-linked immunosorbent assay (ELISA) .
Applications and Validation
The antibody has been validated for:
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Western Blot: Detects OSBPL9 in human brain, testis, and mouse lung tissues at dilutions of 1:500–1:1000 .
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Immunohistochemistry: Stains human testis and colon cancer tissues, requiring antigen retrieval with TE buffer (pH 9.0) or citrate buffer (pH 6.0) .
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ELISA: Used for quantitative detection of OSBPL9 in lysates or sera.
Role of OSBPL9 in Cellular Function
OSBPL9 functions as a cholesterol transfer protein, mediating sterol transport between the endoplasmic reticulum (ER) and trans-Golgi/TGN, ensuring Golgi integrity and protein trafficking . Its depletion via RNAi disrupts Golgi structure and impairs vesicular transport, while overexpression of a dominant-negative variant (ORP9S) halts protein secretion entirely .
Antibody Use in Key Studies
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Lysosomal Repair Pathways: The antibody was used to demonstrate that OSBPL9 interacts with ORP10 to transfer phosphatidylinositol 4-phosphate (PI-4P) at ER-TGN contact sites, facilitating lysosomal membrane repair .
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Golgi Fragmentation: Studies employing this antibody revealed that OSBPL9 knockdown leads to Golgi fragmentation and endosomal cholesterol accumulation, underscoring its role in lipid homeostasis .
Citations and References
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Wyles and Ridgway (2004): Role of ORP9 in ER-Golgi sterol transport.
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Proteintech (2023): OSBPL9 antibody validation data and publications.
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National Library of Medicine (2025): OSBPL9 gene entry (public domain).
Product Specs
Target Background
- ORP9 is identified as a dual sterol/PI-4P binding protein potentially regulating PI-4P within the Golgi apparatus. PMID: 25255026
- The T allele of rs768529 is potentially associated with increased risk of carotid vulnerable plaque formation in the Chinese Hunan Han population. PMID: 24190612
- ORP11 is identified as an OSBP homologue localized at the Golgi-lysosomal endosome interface. The ORP9-ORP11 dimer functions as a potential intracellular lipid sensor or transporter. PMID: 20599956
- N-alpha terminal acetylation of OSBL9_HUMAN is documented, initiating with nASIMEGPLSK after cleavage of the initiating Met-1 residue. PMID: 19413330
- Mammalian target of rapamycin is implicated in ORP9L phosphorylation in HEK293 cells. ORP9 is identified as a PDK-2 substrate and a negative regulator of Akt phosphorylation at the PDK-2 site. PMID: 16962287
- MicroRNA-125a-5p may partially regulate the proinflammatory response, lipid uptake, and ORP9 expression in oxLDL-stimulated monocyte/macrophages. PMID: 19377067
