osta-1 Antibody

Introduction to OSTA-1 Antibody

The OSTA-1 antibody (Product ID: 31330-1-AP) is a rabbit-derived polyclonal antibody validated for Western blot (WB) and ELISA. It recognizes the SLC51A protein, a critical component of the heterodimeric OST-alpha/OST-beta complex, which facilitates bile acid transport across cellular membranes .

Biological Function of SLC51A (OSTA)

SLC51A is essential for bile acid export from enterocytes into portal blood. Key functional insights include:

• Cellular Localization: Resides in the basolateral plasma membrane after interacting with its partner protein SLC51B (OSTB) .

• Tissue Distribution: Highly expressed in the kidney and intestine, with implications for bile acid homeostasis .

• Disease Association: Mutations in SLC51A are linked to cholestasis progressive familial intrahepatic 6 (PFIC6), a rare genetic disorder causing impaired bile flow .

Experimental Protocols

The antibody has demonstrated reactivity in human, mouse, and rat samples, supporting cross-species studies .

Clinical and Mechanistic Insights

While the OSTA-1 antibody itself is not directly used in therapeutics, its target SLC51A has been implicated in:

• Bile Acid Regulation: Dysregulation of SLC51A disrupts bile acid recycling, contributing to cholestatic liver diseases .

• Cancer Research: Although not explicitly studied in oncology, related organic solute transporters are explored for roles in drug resistance .

Comparative Analysis of Antibody Performance

Data from preclinical studies highlight its utility:

• Specificity: No cross-reactivity reported with unrelated proteins in human tissue screens .

• Stability: Suitable for assays requiring high-affinity binding under physiological conditions .

Future Directions

Further research is needed to explore SLC51A’s role in metabolic disorders and its potential as a biomarker for cholestasis. Combinatorial therapies targeting OST-alpha/OST-beta could offer novel interventions for PFIC6 .