otsB Antibody
Description
Definition and Target
The otsB Antibody is a polyclonal rabbit-derived antibody raised against the recombinant E. coli otsB protein (amino acids 1–266). It specifically recognizes the TPP enzyme, a key component of the trehalose biosynthesis pathway, which converts trehalose-6-phosphate to trehalose. This enzyme plays a critical role in bacterial stress responses, carbon metabolism, and osmoprotection .
Key Features of the otsB Protein
| Property | Description |
|---|---|
| Function | Catalyzes the dephosphorylation of trehalose-6-phosphate to trehalose. |
| Biological Role | Involved in osmotic stress adaptation and carbon storage regulation. |
| Disease Relevance | Dysregulation linked to bacterial pathogenesis and metabolic disorders. |
Validated Applications
The otsB Antibody is optimized for:
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Western Blotting (WB): Detects otsB protein expression in bacterial lysates. Optimal dilution for PACO64885 is 1:1000–1:5000 .
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ELISA: Quantifies otsB levels in solution (orb752379’s HRP conjugation enhances signal detection) .
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Immunohistochemistry (IHC) and Immunofluorescence (IF): Localizes otsB in cellular compartments (validated for PACO64885) .
Considerations for Experimental Design
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Specificity: Cross-reactivity with human or other species has not been reported; validate for non-E. coli systems.
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Sensitivity: HRP-conjugated variants (e.g., orb752379) may offer higher sensitivity in ELISA assays compared to unconjugated forms.
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Sample Preparation: Denaturing conditions in WB may affect epitope accessibility; optimize buffer systems for native protein detection.
Product Specs
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
KEGG: ecj:JW1886
STRING: 316385.ECDH10B_2038
