OTUD3 Antibody, HRP conjugated

Functional Role of OTUD3

OTUD3 is a deubiquitinase that hydrolyzes Lys-6- and Lys-11-linked polyubiquitin chains, modulating protein stability. Its roles are context-dependent:

• Tumor Suppression: Stabilizes PTEN in breast cancer, inhibiting PI3K/AKT signaling .

• Oncogenic Activity: Stabilizes ACTN4 in hepatocellular carcinoma (HCC), promoting metastasis .

• Glioma Pathogenesis: Low OTUD3 expression correlates with reduced PTEN levels and poor patient survival .

Key Findings Using This Antibody

Experimental Validation

• Specificity: Recognizes the central region (192-220AA) or full-length OTUD3 (108-398AA) .

• Sensitivity: Detects OTUD3 at concentrations as low as 1:2000 dilution in WB .

• Functional Assays: Validated in xenograft models to study OTUD3’s role in tumor growth and metastasis .

Key Research Insights

• Glioma: OTUD3 mRNA and protein levels are significantly reduced in glioma cells compared to normal astrocytes, correlating with PTEN downregulation and shorter survival .

• HCC: OTUD3 stabilizes ACTN4 via deubiquitination, driving tumor proliferation and metastasis .

• Antibody Utility: Enables precise detection of OTUD3 in diverse cancer models, supporting mechanistic and prognostic studies .

References

1. Role of OTUD3-PTEN axis in glioma progression and survival .

2. OTUD3-ACTN4 signaling in hepatocellular carcinoma metastasis .

3. Aviva Systems Biology product specifications .

4. Antibodies-Online validation data .

5. Qtonics HRP-conjugated antibody details .