PHF20L1 Antibody

Introduction to PHF20L1 Antibody

The PHF20L1 antibody is a specialized immunological tool designed to detect and study the Plant Homeodomain Finger Protein 20-Like 1 (PHF20L1), a Tudor domain-containing protein involved in epigenetic regulation and oncogenesis. This antibody enables researchers to investigate PHF20L1's expression patterns, molecular interactions, and functional roles in diseases such as cancer through techniques like Western blotting, immunohistochemistry (IHC), and immunoprecipitation (IP) . PHF20L1 antibodies are critical for elucidating its role in bridging transcriptional repression complexes (e.g., PRC2 and NuRD) and modulating histone modifications like H3K27me2 .

Key Methodologies Enabled by PHF20L1 Antibodies

Molecular Mechanisms

• PHF20L1 recruits PRC2 and NuRD complexes to repress tumor suppressors (HIC1, KISS1, BRCA1) via H3K27me2 recognition .

• Loss of PHF20L1 disrupts G1–S-phase cell cycle checkpoints, increasing S/G2-phase populations by ~20% .

Technical Validation of PHF20L1 Antibody

• Specificity: Validated via siRNA-mediated knockdown, showing ~80% reduction in PHF20L1 signal in Western blots .

• Subcellular Localization: Localizes to the nucleoplasm and plasma membrane, as confirmed by the Human Protein Atlas .

• Functional Validation: Rescue experiments with siRNA-resistant PHF20L1 restored target gene repression and histone modifications (e.g., H3K27me3) .

Clinical Implications

Future Directions

• Therapeutic Targeting: PHF20L1’s Tudor domain represents a potential drug target to disrupt its interaction with PRC2/NuRD .

• Biomarker Development: PHF20L1 expression could stratify patients for CDK4/6 inhibitor therapies (e.g., palbociclib) .