ABI1 Antibody
Description
ABI1 Antibody: Definition and Molecular Context
ABI1 antibodies are immunoreagents designed to detect and study the ABI1 protein, which regulates actin polymerization, cell adhesion, and tyrosine kinase signaling . These antibodies are widely used in techniques such as Western blot (WB), immunohistochemistry (IHC), and immunoprecipitation (IP) . ABI1 interacts with key proteins like SOS1, EPS8, c-Abl, and WAVE2, forming complexes that influence pathways such as Ras-to-Rac signaling and EGFR activation .
Key Molecular Interactions of ABI1
| Interacting Protein | Functional Role |
|---|---|
| SOS1 | Ras-to-Rac signaling |
| c-Abl | Tyrosine kinase activation |
| WAVE2 | Actin polymerization |
| STAT3/NF-κB | T-cell signaling |
Cancer Biology
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Hepatocellular Carcinoma (HCC): ABI1 overexpression correlates with tumor growth, metastasis, and poor prognosis. Knockdown experiments show reduced proliferation and invasion in HCC cells .
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Prostate and Breast Cancer: ABI1 interacts with androgen receptors (AR) and modulates transcriptional activity, influencing disease progression .
Immune System Regulation
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T-Cell Activation: ABI1 KO models reveal its role in suppressing T-cell exhaustion by regulating Lck/Fyn kinases, with implications for CAR T-cell therapy .
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Cytokine Signaling: ABI1 modulates STAT3 and NF-κB pathways, affecting IL-2 secretion and immune synapse formation .
Respiratory Pathophysiology
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Asthma: ABI1 is upregulated in airway smooth muscle (ASM) cells from asthmatic patients, driving proliferation and airway remodeling .
Validation and Specificity Data
ABI1 antibodies exhibit high specificity across human, mouse, and rat samples. Representative examples include:
Mechanistic Insights
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Actin Dynamics: ABI1 knockdown reduces profilin-1 recruitment to lamellipodia, impairing cell migration .
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Oncogenic Signaling: In HCC, ABI1 enhances SFK/STAT3/NF-κB pathways, promoting metastasis .
Clinical Implications
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Asthma Therapy: ABI1 inhibition attenuates ASM thickening and airway hyperresponsiveness in murine models .
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Cancer Prognostics: High ABI1 expression predicts poor survival in HCC and breast cancer .
Future Directions and Therapeutic Potential
ABI1 antibodies are pivotal for exploring its dual role as a tumor promoter or suppressor, depending on cellular context . Ongoing studies focus on targeting ABI1 to enhance CAR T-cell efficacy or mitigate asthma-related airway remodeling .
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Phosphatase ABI1 and okadaic acid-sensitive phosphatases of the PPP family are negative regulators of salt stress-activated SnRK2.4. PMID: 27297076
- ABI1 acts to inhibit MAPKKK18 kinase activity, but also affects MAPKKK18 protein turnover via the ubiquitin-proteasome pathway. SnRK2.6 kinase also seems to be important for the regulation of MAPKKK18 function. PMID: 26852793
- ABI1 regulates the activity and promotes proteasomal degradation of MKKK18. PMID: 26443375
- ABI1 plays a role in the activation of nuclear genes during the transcription of chloroplast genes. PMID: 25976841
- There is direct cross-talk between C/N and non-canonical ABA signaling pathways, regulated by ABI1, in plants. PMID: 25795738
- In the endodermis, misexpression of the ABA insensitive1-1 mutant protein, which dominantly inhibits abscisic acid (ABA) signaling, leads to a substantial recovery in lateral root (LR) growth under salt stress conditions. PMID: 23341337
- Two dominant-negative mutations in ABA insensitive1 (abi1-1) and abi2-1 greatly reduced ROS production in mitochondria. PMID: 22652060
- ROP11 protects ABI1 phosphatase activity from inhibition by RCAR1/PYL9 and thus negatively regulates abscissic acid signaling. PMID: 22251383
- Research has shed light on the molecular basis for PP2Cs-mediated inhibition of SnRK2s. PMID: 22090030
- A study demonstrates that ABI1 can interact with several PYR/PYL/RCAR family members, that PYR1-ABI1 interaction is rapidly stimulated by ABA, and indicates new SnRK2 kinase-PYR/PYL/RCAR interactions in an emerging model for PYR/PYL/RCAR-mediated ABA signaling. PMID: 19874541
- HAB1 and the related PP2Cs ABI1 and ABI2 interact with OST1 in vivo, and mutations in the corresponding genes strongly affect OST1 activation by ABA. PMID: 19855047
- The consequences of altered abscisic acid (ABA) sensitivity in gray poplar (*Populus x canescens* [Ait.] Sm.) development were examined by ectopic expression of the *Arabidopsis thaliana* mutant *abi1* (for abscisic acid insensitive1) gene. PMID: 19837818
- The direct interaction between SRK2E/OST1 and ABI1 through Domain II plays a critical role in the control of stomatal closure. PMID: 16365038
- ABI1 and ABI2 control fibrillin expression that is involved in mediating ABA-induced photoprotection. PMID: 16571665
- ABI1 works with HAB1 to negatively regulate ABA signaling. PMID: 16798945
- *AtBI1* plays a pivotal role as a highly conserved survival factor during ER stress that acts in parallel to the unfolded protein response pathway. PMID: 18039663
- *abi1-1* acts as a hypermorphic allele, and ABI1 reprograms sensitivity towards ABA in the nucleus. PMID: 18298671
- The regulatory component of abscisic acid (ABA) receptor 1 (RCAR1, At1g01360) was shown to bind ABA, to mediate ABA-dependent inactivation of type 2C protein phosphatases (PP2Cs) ABI1 or ABI2 *in vitro*, and to antagonize PP2C action *in planta*. PMID: 19407143
- ABI1 plays the role of a general signal transducer linking abscisic acid and ethylene biosynthesis as well as signaling pathways to ozone stress tolerance. PMID: 19705149
