At5g06750 Antibody
Description
Introduction: Definition and Overview
The At5g06750 Antibody is a research-grade antibody targeting the Arabidopsis thaliana protein At5g06750 (Uniprot ID: Q84JD5), annotated as a probable protein phosphatase 2C 68. This antibody is primarily used in plant molecular biology to study protein function, phosphorylation regulation, and related cellular processes in Arabidopsis thaliana. Two distinct formats are commercially available: a polyclonal rabbit antibody and a monoclonal mouse antibody, each optimized for specific applications .
Antibody Formats and Production
Key Notes:
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The polyclonal antibody is affinity-purified and validated for ELISA and Western blotting (WB) .
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The monoclonal antibody is part of the AbInsure™ program, guaranteeing performance in WB and IP (immunoprecipitation) applications .
Validated Applications
Performance Metrics:
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ELISA Sensitivity: Achieved a 10,000-fold serum dilution with specific binding to HMGB1-related antigens .
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WB Detection Limit: 0.01–1 ng of target protein in dot blot assays .
Specificity and Cross-Reactivity
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Target Specificity: The monoclonal antibody targets the N terminus of Q84JD5, while the polyclonal antibody binds the full-length recombinant protein .
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Cross-Reactivity: No data on cross-reactivity with homologous proteins in other species are provided.
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Validation Challenges: While not explicitly reported for At5g06750, general antibody validation principles (e.g., KO cell lines, orthogonal methods) are critical to avoid nonspecific binding .
Comparative Analysis of Available Antibody Formats
Research Context and Limitations
The At5g06750 Antibody is primarily used in Arabidopsis research, though specific studies are not detailed in publicly available literature. Its role in phosphatase activity or stress signaling remains inferred from gene annotation . Key limitations include:
Product Specs
Composition: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
