Customize ABI2 Antibody

Description

Definition and Biological Context

The ABI2 antibody is a research tool designed to detect and quantify the Abelson Interactor 2 (ABI2) protein, a regulator of actin cytoskeleton dynamics critical for cell motility, adhesion, and neuronal morphogenesis . ABI2 functions as part of the WAVE complex, enabling Arp2/3-mediated actin polymerization essential for lamellipodia formation and dendritic spine specification . Dysregulation of ABI2 is implicated in cancers, neurological disorders, and drug resistance .

Key Methodologies and Protocols

Western Blot (WB): Optimal dilution ranges from 1:2,000 to 1:6,000 . Validated in cell lines (HEK-293, HeLa, SH-SY5Y) and tissues (brain, testis) .
Immunohistochemistry (IHC): Dilution 1:20–1:200; antigen retrieval recommended using citrate (pH 6.0) or TE buffer (pH 9.0) .
Functional Studies: Used to investigate ABI2’s role in cancer stem cell maintenance, metastasis, and drug resistance .

Oncology

Hepatocellular Carcinoma (HCC): ABI2 overexpression correlates with tumor size, pathological grade, and poor survival. Knockdown suppresses stemness (CD24+/CD133+/CD326+ populations) and sorafenib resistance via MEOX2/KLF4-NANOG axis .
Triple-Negative Breast Cancer (TNBC): Low ABI2 expression promotes metastasis via PI3K/Akt signaling. CBLC E3 ligase mediates ABI2 ubiquitination and degradation .
Prostate Cancer: PIM1 kinase stabilizes ABI2 under hypoxia, enhancing WAVE complex activity and invasiveness .

Neurological Implications

ABI2 deficiency in mice causes dendritic spine abnormalities, impaired neuronal migration, and memory deficits .

Molecular Mechanisms

Actin Regulation: ABI2 recruits MEOX2 to activate KLF4 and NANOG transcription, driving cytoskeletal remodeling .
Drug Resistance: CRISPR screening identifies ABI2 as a key mediator of Temozolomide resistance .

Technical Validation

Assay	Results
WB Specificity	Detects bands at 52–56 kDa in human K562, HeLa, and mouse brain lysates .
IHC Localization	Strong staining in human brain/testis and mouse/rat brain tissues .
Knockdown Validation	siRNA-mediated ABI2 knockdown reduces EMT and invasion in NPC cells .

Comparative Antibody Performance

Antibody Clone	Specificity	Applications
Proteintech 14890	Human, Mouse, Rat; Polyclonal	WB, IHC, CoIP
Abcam ab133593	Human, Rat; Monoclonal (EPR7069)	WB, IHC-P
Boster A04302	Human, Mouse, Rat; Polyclonal	WB, IHC, Flow Cytometry

Future Directions

ABI2 antibodies are pivotal for elucidating its role in cytoskeletal dynamics and therapeutic targeting. Ongoing studies focus on:

Therapeutic Inhibition: Blocking ABI2-MEOX2 interactions in HCC .
Biomarker Development: Correlating ABI2 expression with metastatic potential in prostate and breast cancers .

Product Specs

Buffer

**Preservative:** 0.03% Proclin 300
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Lead Time

Made-to-order (14-16 weeks)

Synonyms

ABI2 antibody; PP2C77 antibody; At5g57050 antibody; MHM17.19 antibody; Protein phosphatase 2C 77 antibody; AtPP2C77 antibody; EC 3.1.3.16 antibody; Protein ABSCISIC ACID-INSENSITIVE 2 antibody; Protein phosphatase 2C ABI2 antibody; PP2C ABI2 antibody

Target Names

ABI2

Uniprot No.

O04719

Target Background

Function

ABI2 Antibody is a repressor of the abscisic acid (ABA) signaling pathway. This pathway regulates numerous ABA responses, including stomatal closure, osmotic water permeability of the plasma membrane (Pos), high light stress, response to glucose, seed germination, and inhibition of vegetative growth. During stomatal closure regulation, ABI2 modulates the inward calcium-channel permeability as well as H(2)O(2) and oxidative burst in response to ABA and dehydration. Additionally, it represses GHR1 and, to some extent, SRK2E/OST1, kinases involved in the regulation of SLAC1-dependent stomatal closure. ABI2 negatively controls fibrillin, which is involved in mediating ABA-induced photoprotection. It may also be implicated in ABA content regulation. Further, ABI2 is involved in acquired thermotolerance of root growth and seedling survival and is required for the Erwinia amylovora harpin-induced (HrpN) drought tolerance. Finally, it plays a role in the hydrotropic response.

Gene References Into Functions

Research suggests that ABI2 plays a role in the activation of nuclear genes during transcription of chloroplast genes. PMID: 25976841
Studies indicate that ABI2 may functionally link stress-regulated control of growth and nitrate uptake and utilization, both energy-expensive processes. PMID: 25943353
AtOZF2 is involved in the abscissic acid and salt stress response through the ABI2-mediated signaling pathway. PMID: 22121246

Database Links

KEGG: ath:AT5G57050

STRING: 3702.AT5G57050.1

UniGene: At.22051

Protein Families

PP2C family

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ABI2 Antibody