pi4k2a Antibody

PI4K2A Antibody Overview

The PI4K2A antibody is a primary reagent used in immunodetection assays to study the localization, expression, and functional roles of PI4K2A. It is widely employed in techniques such as:

• Western blot (WB): To quantify PI4K2A protein levels in cell lysates or tissue extracts.

• Immunofluorescence (IF): To visualize PI4K2A localization in subcellular compartments (e.g., Golgi apparatus, lysosomes).

• ELISA: To measure PI4K2A levels in biological samples.

• Immunohistochemistry (IHC): For tissue-specific expression analysis.

Key Features:

• Host: Typically rabbit or mouse (e.g., rabbit polyclonal antibodies from Thermo Fisher Scientific or Cell Signaling Technology ).

• Reactivity: Human, mouse, rat, and sometimes bovine or pig (e.g., Proteintech’s 15318-1-AP ).

• Immunogen: Synthetic peptides or recombinant PI4K2A protein fragments (e.g., Thermo Fisher’s PA5-15275 targets the N-terminal region ).

Research Applications of PI4K2A Antibodies

PI4K2A antibodies are instrumental in studying its role in:

• Cancer Biology: PI4K2A overexpression correlates with poor prognosis in breast and lung cancers. Antibodies have been used to validate PI4K2A as a therapeutic target, particularly in targeting lysosomal networks (e.g., Pac 1 disrupts PI4K2A/PKR interactions in cancer cells ).

• Lysosomal Repair: PI4K2A antibodies demonstrated its recruitment to damaged lysosomes, where it generates PI4P to mediate repair via ER-lysosome membrane contacts .

• Neurodegeneration: Studies using PI4K2A antibodies linked its deficiency to lipofuscin accumulation and neurodegenerative phenotypes in mice and human patients .

Experimental Validation and Performance

Antibodies are validated through:

• Knockdown/Knockout (KD/KO) Studies: Proteintech’s 15318-1-AP was validated in PI4K2A-KO cells .

• Immunoprecipitation: Used to study PI4K2A interactions with proteins like PKR in cancer models .

• Western Blot: Detects a ~54 kDa band corresponding to PI4K2A (e.g., Cell Signaling’s #5527 ).

Clinical Relevance

Antibody-based research has implicated PI4K2A in:

• Tumor Growth: PI4K2A stabilizes EGFR and regulates angiogenesis .

• Neurodevelopmental Disorders: Mutations in PI4K2A cause severe developmental defects and epilepsy .

• Therapeutic Targeting: Small-molecule inhibitors like Pac 1 disrupt PI4K2A activity, offering potential cancer therapies .