P5CSA Antibody
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
Δ1-pyrroline-5-carboxylate synthetase (P5CS) plays a crucial role in proline biosynthesis, a key process in plant osmoregulation.
Key Research Findings on P5CS Function:
- Light regulates salt-induced transcriptional memory through HY5's role in maintaining H3K4me3 levels at memory genes. Salt-induced transcriptional memory of P5CS1 is linked to sustained H3K4me3 levels during recovery. PMID: 27930298
- Proline metabolism is interconnected with phosphate nutrition. Proline biosynthesis is regulated through cross-talk between abscisic acid (ABA) signaling and phosphate homeostasis, involving PHR1 and PHL1-mediated transcriptional activation of the P5CS1 gene. PMID: 28765275
- FRIGIDA (FRI) enhances drought resistance via its downstream P5CS1 pathway, dependent on its target gene, FLC. PMID: 29175388
- Studies on transgenic tobacco plants expressing the Arabidopsis thaliana P5CS1 gene have evaluated their salt tolerance. PMID: 27055296
- P5CS1 and P5CS2 exhibit similar expression levels and patterns in vegetative and floral shoot apical meristems, as well as in axillary meristems. PMID: 19627555
- Intron-mediated alternative splicing of Arabidopsis P5CS1 is associated with natural variation in proline levels and climate adaptation. PMID: 22615385
- Down-regulation of P5CS1 leads to proline reduction, impaired bolting, and stunted growth. PMID: 18060533
Q&A
Here’s a structured FAQ document for researchers studying the P5CSA (Procollagen 5 Alpha-1) antibody, tailored to academic research scenarios and methodological rigor:
Advanced Research Questions
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How to resolve contradictory P5CSA expression data between idiopathic pulmonary fibrosis (IPF) and liver cirrhosis models?
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Analytical framework:
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Perform tissue-specific ECM fractionation (sequential NaCl/EDTA extraction).
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Quantify soluble vs. insoluble P5CSA pools via ELISA/Western blot correlation.
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Consider cross-reactivity with other collagen isoforms (e.g., COL5A2) using siRNA silencing.
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What computational tools enhance P5CSA network analysis in fibrotic pathways?
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Pipeline:
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Integrate RNA-seq data (COL5A1 expression) with P5CSA IHC spatial patterns using QuPath or Halolink.
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Apply STRING database to map collagen V interactions (e.g., TGF-β1, MMP2).
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How to design a longitudinal study assessing P5CSA as a fibrosis progression biomarker?
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Study design:
Parameter Recommendation Rationale Sampling frequency Every 4 weeks over 6 months Aligns with ECM turnover rates Cohort stratification By serum TIMP-1 levels Controls for TGF-β pathway variability Validation method Mass spectrometry (vs. antibody-based) Confirms isoform-specific detection
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Technical Troubleshooting
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Why does P5CSA antibody show nonspecific nuclear staining in immunofluorescence?
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Solutions:
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Pre-absorb antibody with collagen V-depleted lysate (e.g., using COL5A1 siRNA-treated cells).
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Test alternative fixation (4% PFA for 10 min vs. methanol).
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How to quantify P5CSA in complex biological fluids (e.g., ascites)?
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Workflow:
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Concentrate samples via 10 kDa centrifugal filters.
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Pre-clear with protein A/G beads to reduce IgG interference.
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Use sandwich ELISA with monoclonal capture/detection antibody pairs.
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