pab2 Antibody
Description
Overview of PAB2 Antibody
The PAB2 antibody is a research tool designed to detect and study the poly(A)-binding protein 2 (PAB2), a critical regulator of RNA metabolism and cellular signaling. PAB2, encoded by the PABPN1 gene, is involved in mRNA stability, translation initiation, and stress response pathways such as the MAPK cascade . Its canonical form has a molecular weight of 32.7 kDa, with three isoforms identified in human cells . The antibody is widely used in molecular biology techniques like Western blot, ELISA, and immunoprecipitation to analyze PAB2 expression and function .
Key Findings:
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Genetic Interactions: PAB2 interacts with the let-7 miRNA in C. elegans, influencing stem cell fate regulation .
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Post-Translational Regulation: PAB2 expression is negatively regulated by yeast PAB1 in heterologous systems .
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Epitope Prediction: Advanced tools like PAbFold (based on AlphaFold2) predict linear epitopes for antibody design .
Antibody Development and Validation
Polclonal antibodies against PAB2 are typically generated using recombinant proteins or synthetic peptides. For example:
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A rat polyclonal antibody targeting PAB2 was validated in Western blot, detecting a 27 kDa band in C. elegans lysates .
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A rabbit antibody raised against the N-terminal peptide (codons 5–24) was used to study PAB2 function in yeast .
Validation Metrics:
| Assay | Performance |
|---|---|
| Western Blot | Detects ~27 kDa band in C. elegans lysates |
| ELISA | Sensitive detection of PAB2 in biological samples |
| Immunoprecipitation | Efficiently isolates PAB2 for downstream analysis |
Research Applications
PAB2 antibodies are integral to studies on RNA metabolism and disease mechanisms:
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
Pab2 Antibody: Background Gene References and Functions
- While PABPN1 significantly enhances the activity of its corresponding poly(A) polymerase in vitro, Pab2 exhibited a negligible stimulatory effect on Pla1. PMID: 28096519
- Pab2 collaborates with Rrp6 to negatively regulate Tf2 mRNA accumulation. The absence of Pab2 attenuated the derepression of Tf2 transcription and the increased frequency of Tf2 mobilization resulting from the deletion of abp1. PMID: 28007890
- Nab2 hinders Pab2/Rrp6-mediated decay by competing with Pab2 for polyadenylated transcripts within the nucleus. PMID: 24081329
- A proline-tyrosine nuclear localization signal serves as both a necessary and sufficient condition for nuclear import of Pab2. PMID: 23279110
- Pab2 and the nuclear exosome subunit, Rrp6, are the primary factors involved in this polyadenylation-dependent pre-mRNA degradation pathway. PMID: 21981922
- A model proposes that Mmi1 selectively targets meiotic transcripts for degradation through Pab2 and the exosome. PMID: 20622014
- Pab2 facilitates poly(A) tail trimming from pre-snoRNAs by recruiting the nuclear exosome. PMID: 20129053
KEGG: spo:SPBC16E9.12c
STRING: 4896.SPBC16E9.12c.1
