PAE1 Antibody
Description
Definition and Biological Context
PAGE1 Antibody is an immunoglobulin designed to detect PAGE1, a cancer-testis antigen expressed in prostate and other cancers. It belongs to the GAGE protein family and is encoded by the PAGE1 gene. This 16.2 kDa protein (146 amino acids) is implicated in tumorigenesis and immune evasion .
Antibody Properties
| Parameter | Details |
|---|---|
| Target Epitope | Amino acids 81–130 of human PAGE1 (UniProt ID: O75459) |
| Host Species | Rabbit (polyclonal) |
| Reactivity | Human, Monkey (83–100% homology) |
| Applications | Western Blotting (WB), Immunohistochemistry (IHC), ELISA |
| Conjugate | Unconjugated |
| Immunogen | Synthetic peptide (aa81–130) |
| Purification | Protein A affinity chromatography |
PAGE1 Protein Features
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Function: Associated with tumor progression; classified as a cancer-testis antigen due to restricted expression in normal tissues (e.g., testis) and overexpression in malignancies .
Key Studies
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Expression Analysis: PAGE1 antibodies (e.g., ABIN321041) confirmed PAGE1 overexpression in prostate cancer cell lines via WB .
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Therapeutic Potential: While PAGE1 itself is not directly targeted in current therapies, analogous antibodies against cancer-testis antigens (e.g., GAGE family) are explored for immunotherapy .
Comparative Antibody Performance
| Product (Catalog No.) | Host | Clonality | Applications |
|---|---|---|---|
| ABIN321041 | Rabbit | Polyclonal | WB |
| ab31280 (Anti-PAI1) | Goat | Polyclonal | WB, IHC |
| MEDI-579 (Anti-PAI-1) | Human | Monoclonal | Inhibits serine proteases |
Technical Considerations
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Specificity: Predicted reactivity includes Gorilla (100%) and Marmoset (83%) due to sequence homology .
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Validation: Western blot data show a single band at ~16 kDa in A549, HepG2, and HeLa cell lysates .
Limitations and Future Directions
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Research Gaps: Limited clinical data on PAGE1’s mechanistic role in cancer.
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Opportunities: Development of monoclonal PAGE1 antibodies could enhance diagnostic specificity and therapeutic targeting.
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
