Customize PAE3 Antibody

Product Specs

Buffer

**Preservative:** 0.03% Proclin 300
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Lead Time

Made-to-order (14-16 weeks)

Synonyms

PAE3 antibody; At2g46930 antibody; Pectin acetylesterase 3 antibody; EC 3.1.1.- antibody

Target Names

PAE3

Uniprot No.

O80731

Target Background

Function

This antibody hydrolyzes acetyl esters within homogalacturonan regions of pectin. In type I primary cell walls, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Reducing the degree of acetylation of pectin gels in vitro alters their physical properties.

Database Links

KEGG: ath:AT2G46930

STRING: 3702.AT2G46930.1

UniGene: At.22242

Protein Families

Pectinacetylesterase family

Subcellular Location

Secreted, cell wall.

Q&A

The following FAQs address key methodological considerations for PAGE3 (GAGED1) antibody applications in academic research, synthesized from technical literature and product documentation. Common typographical variations (PAE3 vs. PAGE3) have been resolved using UniProt cross-referencing (Q5JUK9) .

What experimental factors explain contradictory PAGE3 localization reports (nuclear vs. cytoplasmic)?

Advanced troubleshooting matrix:

Observed Localization	Likely Cause	Resolution Strategy
Nuclear predominant	Over-fixation (≥24hr formalin)	Reduce fixation to 6-12hr
Cytoplasmic dominant	Epitope masking by binding partners	Add 0.1% Triton X-100 permeabilization
Diffuse staining	Cross-reactivity with PAGE family paralogs	Validate with CRISPR KO lines

Source contradictions arise from:

Tissue-specific post-translational modifications (phosphorylation at Ser27 alters subcellular distribution)
Antibody lot variations in polyclonal preps (batch-to-batch epitope diversity)

How to design PAGE3 co-immunoprecipitation experiments considering its low molecular weight (12 kDa)?

Optimized workflow:

Crosslinker choice: Use DSP (dithiobis[succinimidyl propionate]) for membrane-associated complexes vs. EGS for nuclear interactions .
Electrophoresis: 16% Tris-Tricine gels to resolve <15 kDa proteins .
Blotting: 0.1 μm PVDF membranes with 20% methanol transfer buffer to retain small proteins.

Critical controls:

Include GAPDH spiking to detect proteolytic degradation
Validate with Silver stain before Western transfer

What orthogonal methods confirm PAGE3 antibody specificity in ELISA?

Validation cascade:

Method	Key Metric	Acceptable Range
Surface plasmon resonance	KD value	≤1 nM
Mass spectrometry	Unique peptide coverage	≥2 peptides spanning aa 45-93
Competitive ELISA	IC50 shift with immunogen peptide	≥100-fold vs. scrambled peptide

Data contradiction resolution: Discrepancies between IHC and ELISA typically stem from conformational epitope requirements. Solution: Use native PAGE for antigen preparation .

How to integrate PAGE3 antibody data with single-cell omics datasets?

Advanced integration pipeline:

Spatial mapping: Align IHC H-scores with Visium HD spatial transcriptomics (10 μm registration) .
Proteogenomic bridging:
- Cluster scRNA-seq data using PAGE3+ epithelial signatures
- Perform CITE-seq with TotalSeq™-labeled PAGE3 antibody
Validation loop: Use antibody-derived tags to refine pseudotime trajectories in SCENIC analyses.

Key challenge: Antibody signal decay (t1/2 = 8.3hr in live-cell imaging) requires synchronization with scRNA-seq timepoints .

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PAE3 Antibody