Patatin-15 Antibody

Basic Research Questions

• What is Patatin-15 and why would researchers need an antibody against it?

  Patatin-15 is one of several patatin isoforms found in Solanum tuberosum (potato). Patatins represent approximately 60% of the total soluble protein in potato tubers and function as storage proteins with additional lipolytic acyl hydrolase (LAH) activity . These proteins are dimers composed of 40-42 kDa subunits without disulfide bridges .

  Researchers need Patatin-15 antibodies for various applications including:

  • Studying protein storage mechanisms in plant biology

  • Investigating plant defense responses (patatins are thought to be involved in responses to pathogens)

  • Examining the evolutionary relationships of patatin-like phospholipases across species

  • Analyzing patatin expression patterns during tuber development

• What are the validated applications for Patatin-15 antibody?

  According to available data, Patatin-15 antibodies have been validated for several laboratory applications:

  Application	Validation Status	Notes
  ELISA	Validated	Commonly used for quantification
  Western Blot	Validated	Typically detects bands at ~42 kDa
  Immunoprecipitation	Validated	Useful for protein complex analysis
  Immunolocalization	Validated	For cellular/tissue distribution studies

  When selecting a Patatin-15 antibody, researchers should verify the specific applications validated by the manufacturer for their particular antibody clone or lot .

• What species reactivity can be expected with Patatin-15 antibody?

  Commercially available Patatin-15 antibodies are primarily designed to react with Solanum tuberosum (potato) proteins . Cross-reactivity with patatin-like proteins from other Solanum species may occur but should be experimentally verified. Due to the evolutionary conservation of the patatin domain, some antibodies might recognize patatin-like phospholipases in other plant species, but this would depend on epitope conservation and should not be assumed without validation .

  For researchers working with plant pathogens, it's important to note that some patatin-like domains exist in microbial proteins, but specific anti-Patatin-15 antibodies are unlikely to cross-react without confirmation .

• How should Patatin-15 antibody be stored for optimal stability?

  Based on manufacturer recommendations, Patatin-15 antibodies require careful storage considerations:

  • Upon receipt, store at -20°C or -80°C to maintain long-term stability

  • Avoid repeated freeze-thaw cycles, which can degrade antibody quality and reduce binding efficiency

  • For polyclonal preparations often supplied in stabilizing buffers (e.g., 50% glycerol, 0.01M PBS, pH 7.4), aliquoting before freezing is recommended

  • Working stocks can typically be maintained at 4°C for 1-2 weeks during active use periods

  Always refer to the specific storage conditions recommended by the manufacturer for the particular antibody preparation you are using.

• What immunogen strategies are used to generate Patatin-15 antibodies?

  Patatin-15 antibodies are typically generated using one of these immunogen approaches:

  • Recombinant full-length Patatin-15 protein

  • Synthetic peptides derived from specific regions of Patatin-15

  • KLH-conjugated synthetic peptides derived from conserved C-terminal regions of potato patatin isoforms (including Q3YJS9, Q3YJT0, Q42502, Q3YJT2)

  The choice of immunogen affects epitope recognition and potentially cross-reactivity with other patatin isoforms. Antibodies raised against conserved regions may detect multiple patatin variants, which can be advantageous for general patatin detection but potentially problematic for studies requiring isoform specificity .