PBL20 Antibody
Product Specs
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
Target Background
Q&A
The PBL20 antibody is a polyclonal antibody raised against recombinant Arabidopsis thaliana PBL20 protein, primarily used in research applications like Western blotting (WB) and ELISA . Below is a structured FAQ addressing common technical and methodological challenges in academic research contexts:
Advanced Methodological Challenges
How to resolve inconsistent PBL20 antibody performance across experiments?
What orthogonal methods confirm PBL20 antibody reliability in protein-protein interaction studies?
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Co-immunoprecipitation (Co-IP): Pair with a tagged interactor (e.g., FLAG-RIN4) and validate using mass spectrometry.
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Microscopy: Use transient expression in Nicotiana benthamiana with fluorescent markers to corroborate subcellular localization.
Troubleshooting Data Contradictions
How to address discrepancies between ELISA and Western blot results?
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Epitope accessibility: ELISA detects linear epitopes, while WB depends on tertiary structure. Confirm denaturation conditions in WB .
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Quantitative vs. qualitative: Use ELISA for concentration measurements and WB for size validation.
Why does PBL20 antibody show non-specific bands in root tissue extracts?
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Cross-reactivity: Pre-absorb the antibody with root-specific protein extracts.
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Post-translational modifications: Treat samples with phosphatases/kinases to rule out phosphorylation-dependent epitopes.
