PCBP1 Antibody
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Description
Overview of PCBP1 Antibody
The PCBP1 antibody is a polyclonal immunoglobulin (IgG) raised in rabbits, designed to specifically target the Poly(rC)-binding protein 1 (PCBP1), a key RNA-binding protein involved in post-transcriptional gene regulation . PCBP1 is characterized by its three KH (K homology) domains, which enable binding to poly(C) motifs in RNA, stabilizing mRNA and regulating translation . The antibody is widely used in molecular biology research to detect PCBP1 in human, mouse, and rat samples via techniques such as Western blotting (WB), immunoprecipitation (IP), and immunohistochemistry (IHC) .
Key Features:
3.1. Western Blotting
The antibody detects a single band at ~35 kDa in WB, corresponding to PCBP1 . It is commonly used to study PCBP1 expression in cancer immunity, where PCBP1 stabilizes effector T-cell functions by restricting regulatory T-cell differentiation .
3.2. Immunoprecipitation (IP)
Used to isolate PCBP1 from cell lysates, facilitating downstream analysis of its RNA-binding partners or associated proteins (e.g., cGAS in innate immunity) .
3.3. Immunohistochemistry (IHC)
Localizes PCBP1 in tissue sections, aiding studies of its subcellular distribution (e.g., nuclear vs. cytoplasmic localization in immune cells) .
4.1. Role in Cancer Immunity
PCBP1 was identified as an intracellular immune checkpoint that prevents effector T-cell exhaustion by repressing regulatory T-cell programs . Antibody-based detection confirmed that PCBP1 deletion in T cells enhances tumor growth and reduces antitumor immunity .
4.2. Innate Antiviral Response
The antibody revealed PCBP1’s interaction with cGAS, a key DNA sensor, enhancing its ability to detect cytosolic DNA and activate downstream immune signaling (e.g., IFN-β production) . PCBP1 deficiency impaired cGAS-mediated responses to herpes simplex virus (HSV-1), increasing viral replication .
4.3. Post-Transcriptional Regulation
PCBP1 stabilizes mRNAs with C-rich motifs, such as those encoding α-globin and tyrosine hydroxylase . The antibody has been used to study its role in translational silencing during cell differentiation (e.g., 15-lipoxygenase mRNA) .
Product Specs
Buffer
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. Store at -20°C. Avoid freeze / thaw cycles.
Lead Time
Typically, we can ship the products within 1-3 business days after receiving your order. The delivery time may vary depending on the purchasing method or location. Please consult your local distributors for specific delivery time information.
Synonyms
Alpha-CP1 antibody; Heterogeneous nuclear ribonucleoprotein E1 antibody; heterogenous nuclear ribonucleoprotein E1 antibody; heterogenous nuclear ribonucleoprotein X antibody; hnRNP E1 antibody; hnRNP-E1 antibody; hnRNP-X antibody; HNRPE1 antibody; HNRPX antibody; nucleic acid binding protein sub 2.3 antibody; Nucleic acid- binding protein SUB2.3 antibody; Nucleic acid-binding protein SUB2.3 antibody; PCBP1 antibody; PCBP1_HUMAN antibody; poly(rC) binding protein 1 antibody; Poly(rC)-binding protein 1 antibody; rCbinding protein 1 antibody
Target Names
PCBP1
Uniprot No.
Target Background
Function
PCBP1 is a single-stranded nucleic acid binding protein with a preference for binding to oligo dC. In cases of poliovirus infection, PCBP1 plays a crucial role in initiating viral RNA replication in collaboration with the viral protein 3CD.
Gene References Into Functions
- Knockdown of PCBP1 promotes both tumorigenesis and metastatic progression in prostate cancer, suggesting that PCBP1 acts as a tumor suppressor in normal prostate epithelial cells and contributes to tumor formation by up-regulating MAPK1 expression. PMID: 30078000
- PCBP1 binds to severely oxidized RNA to activate apoptosis-related reactions. While PCBP1 does not bind to oligoribonucleotides with two 8-oxoG residues located far apart, it binds firmly to oligoribonucleotides where these residues are located in proximity. The structure-function relationship of PCBP1 was established using PCBP1 mutant proteins with defective conserved KH domains. PCBP1 functions when RNA is severely damaged. PMID: 29891675
- High HNRPE1 expression is associated with pancreatic cancer metastasis. PMID: 28560430
- UBE4A targets PCBP1 for proteasomal degradation, and UBE4A expression can serve as a novel biomarker in thyroid cancer patients. PMID: 28963376
- A study reports a novel interaction between mutant HSPB1-P182L and the RNA binding protein PCBP1, leading to a reduction in its translational repression activity. Identifying PCBP1 mRNA targets revealed a significant prevalence for an RNA recognition motif, particularly observed in their 5' and 3'UTRs. These findings further support a role for mutant HSPB1 in neurodegenerative diseases. PMID: 28077174
- Research indicates that TGF-beta directly regulates alternative splicing of the cancer stem cell marker CD44 through a phosphorylated threonine179 of SMAD3-mediated interaction with RNA-binding protein PCBP1. PMID: 27746021
- Studies suggest that PCBP1 is a molecular marker of oxaliplatin resistance in colorectal cancer. PMID: 28076324
- A novel positive feedback loop amplifies hnRNP-E1 during prolonged folate deficiency, maximizing the upregulation of folate receptors to restore folate homeostasis to normalcy in placental cells. PMID: 28250194
- TGF-beta signaling through Akt2 induces phosphorylation of heterogeneous nuclear ribonucleoprotein E1 (hnRNP E1) at serine-43 (p-hnRNP E1), driving epithelial to mesenchymal transition and metastasis. PMID: 27067543
- Findings suggest that a coordinated effort among TIA1, Pcbp1, and RBM39 stabilizes or increases U2 snRNP recruitment, enhances spliceosome A complex formation, and facilitates exon definition through RBM39-mediated splicing regulation. PMID: 28193846
- BC200 RNA interacts with hnRNP E1 and E2 primarily through its unique 3' C-rich domain. PMID: 28027391
- Low expression of PCBP1 is associated with lung cancer metastasis. PMID: 27683057
- miR-7977 in extracellular vesicles may be a crucial factor that induces failure of normal hematopoiesis via poly(rC) binding protein 1 suppression in myeloid neoplasms. PMID: 26802051
- Poly C binding protein 1 represses autophagy through downregulation of LC3B to promote tumor cell apoptosis in starvation. PMID: 26880484
- Inhibin betaA is translationally regulated by TGFbeta via hnRNP E1. PMID: 26096938
- alphaCP1/ alphaCP2 binding to a cytosine-rich subset of polypyrimidine tracts drives a novel pathway of cassette exon splicing in the mammalian transcriptome. PMID: 26896798
- Research suggests that PCBP1 might play a significant role in preventing the process of epithelial mesenchymal transformation in NSCLC. PMID: 26261610
- PCBP1 mutations are recurrent in Burkitt lymphomas and might contribute, in collaboration with other mutations, to its pathogenesis. PMID: 26173642
- Findings provide mechanistic evidence for HOTAIR overexpression and PCBP1 downregulation in gastric cancer metastasis and their correlation with poor survival. PMID: 25612617
- Proteomic profiling and subsequent validation indicate that PCBP1 is central to cancer stem cells enrichment and functionality in prostate cancer. PMID: 25661993
- PCBP1 is a central player in the molecular mechanism causing EMT in GBC-SD cells after TGF-beta treatment; PCBP1 might serve as a useful biomarker for predicting the prognosis of gallbladder cancer metastasis. PMID: 24889597
- hnRNP E1 is a new regulator of protein disulphide isomerase translation in oxLDL-activated vascular endothelial cells. PMID: 25389050
- A novel mechanism by which POLH expression is controlled by PCBP1 via mRNA stability. PMID: 25268038
- Reduced expression of hnRNP-E1 might be involved in the cervical cancer pathogenesis, with folate playing a role in the natural history of HPV infection. PMID: 24848140
- The SchA-FAK-p85 complex subsequently selectively recruited and activated Akt2, not Akt1. PMID: 24819169
- PCBP1 is a positive regulator involved in regulating EV71 replication in the host specialized membrane-associated replication complex. PMID: 24489926
- PCBP1 and PCBP2 may serve as iron chaperones to multiple classes of cytosolic nonheme iron enzymes and may play a specific role in restoring metal cofactors that are spontaneously lost in iron-deficient cells. PMID: 24843120
- Poly(rC)-binding protein 1 indirectly regulated MAP2K2, FOS, FST, TP53, and WNT7B through H2AFX. PMID: 22622828
- PCBP1 regulates p63 expression via mRNA stability. PMID: 23940783
- High hnRNP-E1 expression is associated with uremic patients on hemodialysis. PMID: 23439585
- PCBP1 and -2 form a complex for iron delivery to ferritin, and all PCBPs may share iron chaperone activity. PMID: 23640898
- PCBP1 plays a role in posttranscriptional control pathways that can alter the coding potential and/or levels of expression of subsets of mRNAs in the transcriptome. PMID: 23629627
- Down-regulation of poly(rC)-binding protein 1 correlates with the malignant transformation of hydatidiform moles. PMID: 22801034
- A study investigated the PCBP1 KH domain recognition of C-rich nucleotides. X-ray diffraction data of PCBP1 were collected to 1.77 Angstrom resolution and the diffraction was consistent with space group P2(1), with unit-cell parameters a = 38.59, b = 111.88, c = 43.42 A, alpha = gamma = 90.0, beta = 93.37 degrees. The unit-cell volume is consistent with the presence of four protein-DNA complexes in the asymmetric unit. PMID: 22102042
- Findings unravel a critical role of PCBP1 in regulating MAVS for both fine-tuning the antiviral immunity and preventing inflammation. PMID: 22105485
- In HCC patients, the expression of THAP11 mRNA significantly correlated with PCBP1 mRNA expression. These results suggest a novel role of THAP11 in CD44 alternative splicing and hepatoma invasion. PMID: 22673507
- hnRNP-E1 is a physiologically relevant, sensitive, cellular sensor of folate deficiency. PMID: 21930702
- Overall, the results presented suggest that cellular PCBP2 and PCBP1 antagonize vesiculovirus growth by affecting viral gene expression, highlighting the importance of these two cellular proteins in restricting virus infections. PMID: 21752917
- PCBP1 plays a significant role in the dynamic expression of AR in both male and female androgen-responsive cells. PMID: 20519371
- PCBP1 has been characterized as a negative regulator of CD44 variants splicing in HepG2 cells. PMID: 20361869
- Loss of PRL-3 is associated with cancer. PMID: 20609352
- The binding of HuR, CP1, and CP2 to AR mRNA suggests a role for each of these proteins in the post-transcriptional regulation of AR expression in cancer cells. PMID: 12011088
- PCBP1, a highly homologous isoform of PCBP2, binds to poliovirus stem-loop I with an affinity similar to that of PCBP2. PMID: 12414943
- The 3'-untranslated region of p21WAF1 mRNA is a composite cis-acting sequence bound by RNA-binding proteins from breast cancer cells, including PCBP1. PMID: 12431987
- HADHB trifunctional enzyme, human renin, and poly(C)-binding protein are novel renin mRNA-binding proteins that target a cis-element in the 3'-UTR of renin mRNA and regulate renin production. PMID: 12933794
- Deletion of NLS I from alphaCP1 blocks its nuclear accumulation, while both NLS I and NLS II must be inactivated to block nuclear accumulation of alphaCP2. PMID: 14612387
- The interactions of PTB-1 and PCBP1 with their cognate binding sites on the Bag-1 IRES disrupt many of the RNA-RNA interactions, creating a largely unstructured region of approximately 40 nucleotides that could permit ribosome binding. PMID: 15169918
- Kaposi's sarcoma-associated herpesvirus ORF57 binds to PCBP1 as a functional partner for posttranscriptional regulation and is involved in regulating the expression of both cellular and viral genes through IRESs. PMID: 15246275
- hnRNPE1 is a positive effector of collagen synthesis acting at the post-transcriptional level by interaction with the 3'-untranslated regions (3'-UTRs) of COL1A1, 1A2 mRNAs. PMID: 15514164
- PCBPs act as a transcription factor and positively regulate mu opioid receptor gene expression in NMB cells. PMID: 15933215
Database Links
Subcellular Location
Nucleus. Cytoplasm. Note=Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm.
Tissue Specificity
Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands.
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