PCDH17 Antibody, Biotin conjugated

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Description

Introduction to PCDH17 Antibody, Biotin Conjugated

PCDH17 (Protocadherin 17) is a cell adhesion molecule involved in neuronal development and synaptic plasticity. The PCDH17 Antibody, Biotin conjugated is a specialized immunoreagent where a biotin molecule is covalently attached to the antibody, enabling high-affinity binding to streptavidin or avidin. This conjugation enhances signal detection in assays like ELISA, Western blot (WB), and immunohistochemistry (IHC), leveraging the biotin-avidin interaction for amplified sensitivity .

Structure and Conjugation Mechanism

The biotin-conjugated PCDH17 antibody retains the specificity of the original antibody while gaining versatility through biotinylation. Key features include:

AspectDetails
Target ProteinPCDH17 (1159 aa, ~126 kDa calculated; observed 150–170 kDa)
Host/IsotypePolyclonal rabbit or goat IgG (depending on supplier)
Conjugation SiteBiotin attached to lysine residues or other reactive groups on the antibody
ApplicationsELISA, IHC, WB, affinity purification, and signal amplification assays

The biotin moiety enables compatibility with streptavidin-conjugated enzymes (e.g., HRP, AP) or fluorophores, allowing flexible detection strategies .

ELISA and Signal Amplification

Biotin-conjugated PCDH17 antibodies are critical in ELISA protocols for quantifying PCDH17 levels. The biotin-streptavidin interaction amplifies signals, improving assay sensitivity. For example:

  • Detection Protocol: Biotinylated primary antibody binds PCDH17, followed by streptavidin-HRP conjugate addition. TMB substrate generates a colorimetric signal proportional to PCDH17 concentration .

  • Interference Considerations: High endogenous biotin levels in samples (e.g., freeze-dried egg yolks) may falsely elevate signals, necessitating blocking agents or alternative detection methods .

Immunohistochemistry (IHC)

In IHC, biotin-conjugated antibodies enable precise localization of PCDH17 in tissue sections. Streptavidin-linked markers (e.g., Alexa Fluor dyes) enhance fluorescence detection:

  • Example Workflow:

    1. Tissue fixation and blocking.

    2. Incubation with biotin-conjugated PCDH17 antibody.

    3. Streptavidin-fluorophore conjugate addition.

    4. Visualization via microscopy .

Western Blot (WB)

While less common for biotin-conjugated antibodies, WB applications leverage streptavidin-HRP for enhanced band detection:

  • Observed Bands: ~150–170 kDa in mouse/rat brain lysates and human tissues .

  • Dilution Recommendations: 1:500–1:1000 for unconjugated antibodies; biotin-conjugated variants may require optimization .

Advantages of Biotin Conjugation

AdvantageEvidence
Signal AmplificationBiotin-streptavidin interaction increases assay sensitivity by 30-fold .
VersatilityCompatible with multiple streptavidin conjugates (HRP, fluorophores, beads) .
Enrichment EfficiencyAnti-biotin antibodies enable targeted peptide enrichment from complex mixtures .

Challenges and Mitigation Strategies

ChallengeSolution
Biotin InterferenceUse non-biotin blocking agents or replace streptavidin with anti-biotin antibodies .
Cross-ReactivityValidate antibody specificity with WB or ELISA controls .
Optimal DilutionTitrate antibody concentrations to avoid background noise .

Product Overview

SupplierCatalog #ConjugateApplicationsQuantityPrice
Bioss Inc.-BiotinELISA, IHC100 µl$447.00
Creative BiolabsCBMOAB-91644FYABiotinWB, ELISAInquireInquire

Data sourced from Biocompare listings .

Key Considerations

  • Reactivity: Primarily validated for human, mouse, and rat samples .

  • Storage: -20°C in PBS with sodium azide/BSA .

  • Purity: Antigen-affinity purified to ensure specificity .

Product Specs

Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Typically, we can ship your order within 1-3 business days of receipt. Delivery times may vary depending on the purchasing method and location. Please consult your local distributor for specific delivery information.
Synonyms
Protocadherin-17 (Protocadherin-68), PCDH17, PCDH68 PCH68
Target Names
PCDH17
Uniprot No.

Target Background

Function
PCDH17 is a protein that plays a potential role in calcium-dependent cell adhesion.
Gene References Into Functions
  1. Methylation of PCDH17 may play a significant role in the development and progression of high-grade serous ovarian carcinoma (HGSOC) and could potentially serve as a target for new clinical biomarkers. PMID: 29991130
  2. PCDH17 methylation in serum has been identified as a potential prognostic biomarker for patients with renal cell carcinoma after surgery. PMID: 28688232
  3. PCDH17 functions as a tumor suppressor, inhibiting Wnt/beta-catenin signaling and metastasis in breast cancer. However, frequent methylation of PCDH17 in primary tumors suggests its potential as a biomarker. PMID: 27351130
  4. Aberrant methylation of protocadherin 17 has been linked to acute lymphoblastic leukemia. PMID: 27643535
  5. PCDH-17 has been shown to inhibit metastasis through the EGFR/MEK/ERK signaling pathway. PMID: 26386721
  6. Combined methylation analysis of POU4F2/PCDH17 has demonstrated the highest sensitivity (90.00%) and specificity (93.96%) in detecting bladder cancer across diverse sample groups. This finding suggests the potential of this combination for effective bladder cancer detection. PMID: 26700620
  7. PCDH17 methylation in serum is a common occurrence in early-stage prostate cancer, and it is an independent predictor of biochemical recurrence (BCR) after radical prostatectomy. PMID: 26683656
  8. PCDH17 methylation has been observed more frequently in clear cell renal cell carcinoma patients and is associated with malignant clinicopathological characteristics and poor prognosis. PMID: 26404644
  9. PCDH17 promoter methylation has a strong association with bladder cancer malignancy and can be used as an independent predictor of clinical outcomes in patients with bladder cancer. PMID: 24567353
  10. PCDH17 promoter methylation has been shown to be significantly associated with malignant behavior and poor prognosis in bladder cancer patients. PMID: 24366498
  11. This study highlights the crucial role of PCDH17 in the synaptic development of specific corticobasal ganglia circuits and suggests its involvement in these circuits in depressive behaviors. PMID: 23684785
  12. PCDH17 acts as a tumor suppressor, inhibiting proliferation by inducing apoptosis and autophagy. It is frequently silenced in gastric and colorectal cancers. PMID: 22926751
  13. Research indicates that PCDH17 is transcriptionally downregulated in gastric cancer due to aberrant promoter CpG island methylation. PMID: 22207556
  14. Statistical analysis revealed significant downregulation of PCDH17/PCH68 and PTPRD. PMID: 21213369
  15. Findings suggest that silencing of PCDH17 expression through promoter hypermethylation or other mechanisms leads to the loss of its tumor-suppressive activity, potentially contributing to the development of a subgroup of ESCCs. PMID: 20200074
  16. Azoospermic testis exhibited down-regulation of CDH18 and PCDH17. PMID: 20180417
  17. The study discusses the occurrence of an unusual TG 3' splice site in intron 2. PMID: 17672918

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Database Links

HGNC: 14267

OMIM: 611760

KEGG: hsa:27253

UniGene: Hs.106511

Subcellular Location
Cell membrane; Single-pass type I membrane protein.

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