PCDH17 (Protocadherin 17) is a cell adhesion molecule involved in neuronal development and synaptic plasticity. The PCDH17 Antibody, Biotin conjugated is a specialized immunoreagent where a biotin molecule is covalently attached to the antibody, enabling high-affinity binding to streptavidin or avidin. This conjugation enhances signal detection in assays like ELISA, Western blot (WB), and immunohistochemistry (IHC), leveraging the biotin-avidin interaction for amplified sensitivity .
The biotin-conjugated PCDH17 antibody retains the specificity of the original antibody while gaining versatility through biotinylation. Key features include:
The biotin moiety enables compatibility with streptavidin-conjugated enzymes (e.g., HRP, AP) or fluorophores, allowing flexible detection strategies .
Biotin-conjugated PCDH17 antibodies are critical in ELISA protocols for quantifying PCDH17 levels. The biotin-streptavidin interaction amplifies signals, improving assay sensitivity. For example:
Detection Protocol: Biotinylated primary antibody binds PCDH17, followed by streptavidin-HRP conjugate addition. TMB substrate generates a colorimetric signal proportional to PCDH17 concentration .
Interference Considerations: High endogenous biotin levels in samples (e.g., freeze-dried egg yolks) may falsely elevate signals, necessitating blocking agents or alternative detection methods .
In IHC, biotin-conjugated antibodies enable precise localization of PCDH17 in tissue sections. Streptavidin-linked markers (e.g., Alexa Fluor dyes) enhance fluorescence detection:
Example Workflow:
While less common for biotin-conjugated antibodies, WB applications leverage streptavidin-HRP for enhanced band detection:
Observed Bands: ~150–170 kDa in mouse/rat brain lysates and human tissues .
Dilution Recommendations: 1:500–1:1000 for unconjugated antibodies; biotin-conjugated variants may require optimization .
Supplier | Catalog # | Conjugate | Applications | Quantity | Price |
---|---|---|---|---|---|
Bioss Inc. | - | Biotin | ELISA, IHC | 100 µl | $447.00 |
Creative Biolabs | CBMOAB-91644FYA | Biotin | WB, ELISA | Inquire | Inquire |
Data sourced from Biocompare listings .