Applications : Immunohistochemistry (IHC)
Sample type: Paraffin embedded tissue sections of human oral squamous cell carcinoma (OSCC) grade II
Sample dilution: 1:100
Review: After dewaxing and rehydration, tissues were undergone to heat mediated antigen retrieval in citrate buffer pH 6.0. PCNA antibodies were diluted 1:100 and incubated with sections for 12 h at 4 C. DAKO Envision system was used for further reactions.