PDCD7 Antibody

Introduction to PDCD7 Antibodies

PDCD7 antibodies target the PDCD7 protein, encoded by the PDCD7 gene (NCBI Gene ID: 10081). This 55 kDa protein is associated with the U11/U12 small nuclear ribonucleoprotein (snRNP) complex and regulates mRNA splicing via the minor spliceosome . PDCD7 also modulates apoptosis in T-cells and other cell types, with implications in cancer, viral infections, and autoimmune diseases .

Apoptosis Regulation

• PDCD7 overexpression induces apoptosis in lung adenocarcinoma (A549) cells by upregulating pro-apoptotic proteins Bax and caspase-3 while downregulating anti-apoptotic Bcl-2 .

• In H9N2 influenza infection, PDCD7 interacts with the viral PA subunit, amplifying apoptosis via nuclear translocation .

Autoimmune Diseases

• Autoantibodies against PDCD7 are detected in 25% of patients with minor spliceosome-related autoimmune conditions, suggesting epitope spreading within the U11/U12 snRNP complex .

Western Blot Performance

• Proteintech’s 12485-1-AP: Detects a 55 kDa band in HeLa, Jurkat, and MCF7 lysates .

• Abcam’s ab248393: Validated in HeLa, Jurkat, MCF7, and Caco-2 cells, showing consistent 55 kDa bands .

• Thermo Fisher’s MA5-42671: Confirmed reactivity in TE-1, SKOV3, and rodent tissues .

Immunohistochemistry (IHC)

• Sigma-Aldrich’s HPA049388: Stains PDCD7 in human kidney, brain, breast, and colon tissues at 1:200–1:500 dilution .

Mechanistic Studies

• PDCD7 knockdown attenuates PA subunit-induced apoptosis in H9N2-infected cells, confirming its pro-apoptotic role .

• In lampreys, PDCD7 homologs regulate immune responses to pathogens, highlighting evolutionary conservation .

Therapeutic Potential

• PDCD7 inhibitors are explored for neuroprotection in traumatic brain injury (TBI) by modulating NOX2-ROS pathways .

• Targeting PDCD7-autoantibody interactions may benefit autoimmune therapy .