PDGFRA Antibody
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Description
Overview of PDGFRA and Its Antibody
PDGFRA, encoded by the PDGFRA gene, binds platelet-derived growth factor (PDGF) ligands, triggering signaling pathways that regulate proliferation, migration, and survival in mesenchymal cells . Mutations in PDGFRA are implicated in cancers such as gastrointestinal stromal tumors (GISTs) and idiopathic hypereosinophilic syndrome . The PDGFRA antibody facilitates visualization and quantification of the receptor in tissues, enabling research into its normal and pathological functions.
Applications of PDGFRA Antibody
Research Findings and Clinical Relevance
Cancer Associations:
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Mutations in PDGFRA drive oncogenic signaling in GISTs and hypereosinophilic syndromes .
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PDGFRA overexpression correlates with tumor aggressiveness in glioblastoma and melanoma .
Therapeutic Implications:
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PDGFRA inhibitors (e.g., imatinib) target receptor activation in GISTs .
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Antibodies like APA5 (Thermo Fisher) enable precise receptor quantification for drug development .
Developmental Insights:
Comparative Analysis of PDGFRA Antibodies
| Antibody Type | Source | Applications | Species Reactivity | Dilution Range |
|---|---|---|---|---|
| Polyclonal (bs-10989R) | Rabbit | WB, IHC, ELISA | Human, mouse, rat | 1:300–5000 (WB) |
| Monoclonal (APA5) | Mouse | Flow cytometry | Mouse | 0.25 µg/test |
This table highlights the diversity of PDGFRA antibodies, emphasizing their utility across species and experimental platforms.
Product Specs
Buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form
Liquid
Lead Time
Typically, we can ship products within 1-3 business days of receiving your order. Delivery time may vary depending on the purchasing method or location. Please consult your local distributors for specific delivery times.
Synonyms
Alpha-type platelet-derived growth factor receptor antibody; CD140 antigen-like family member A antibody; CD140a antibody; CD140a antigen antibody; MGC74795 antibody; PDGF alpha chain antibody; PDGF Receptor alpha antibody; PDGF-R-alpha antibody; PDGFR 2 antibody; PDGFR alpha antibody; PDGFR2 antibody; PDGFRA antibody; PDGFRA/BCR fusion antibody; PGFRA_HUMAN antibody; Platelet derived growth factor receptor 2 antibody; Platelet derived growth factor receptor alpha antibody; Platelet derived growth factor receptor alpha polypeptide antibody; Platelet derived growth factor receptor antibody; Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein antibody; RHEPDGFRA antibody
Target Names
Uniprot No.
Target Background
Function
The Platelet-Derived Growth Factor Receptor Alpha (PDGFRA) is a tyrosine-protein kinase that functions as a cell-surface receptor for PDGFA, PDGFB, and PDGFC. It plays a pivotal role in regulating embryonic development, cell proliferation, survival, and chemotaxis. Depending on the specific cellular context, PDGFRA can either promote or inhibit cell proliferation and migration. It is essential for the normal development of the skeleton, cephalic closure during embryogenesis, and the differentiation of bone marrow-derived mesenchymal stem cells. Furthermore, PDGFRA is crucial for the development of the gastrointestinal mucosal lining and the recruitment of mesenchymal cells, contributing to the normal development of intestinal villi. It also plays a role in cell migration and chemotaxis during wound healing. In platelets, PDGFRA participates in activation, secretion of agonists from platelet granules, and thrombin-induced platelet aggregation.
Binding of its cognate ligands - homodimeric PDGFA, homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB, or homodimeric PDGFC - leads to the activation of several signaling cascades. The cellular response to ligand binding is dependent on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. PDGFRA phosphorylates PIK3R1, PLCG1, and PTPN11. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+), and activation of protein kinase C. PDGFRA also phosphorylates PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, mediating the activation of the AKT1 signaling pathway. It further promotes the activation of HRAS and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Additionally, PDGFRA stimulates the activation of STAT family members STAT1, STAT3, and STAT5A and/or STAT5B. The receptor signaling is downregulated by protein phosphatases that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor.
Binding of its cognate ligands - homodimeric PDGFA, homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB, or homodimeric PDGFC - leads to the activation of several signaling cascades. The cellular response to ligand binding is dependent on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. PDGFRA phosphorylates PIK3R1, PLCG1, and PTPN11. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+), and activation of protein kinase C. PDGFRA also phosphorylates PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, mediating the activation of the AKT1 signaling pathway. It further promotes the activation of HRAS and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Additionally, PDGFRA stimulates the activation of STAT family members STAT1, STAT3, and STAT5A and/or STAT5B. The receptor signaling is downregulated by protein phosphatases that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor.
Gene References Into Functions
- Hepatic stellate cells release PDGFRalpha-enriched extracellular vesicles. Alcoholic liver disease patients exhibit an increase in PDGFRalpha enrichment within their serum extracellular vesicles. PMID: 29360139
- In addition to replicating a previously identified genome-wide significant locus for corneal astigmatism near the PDGFRA gene, gene-based analysis identified three novel candidate genes, CLDN7, ACP2, and TNFAIP8L3, which warrant further investigation to understand their role in the pathogenesis of corneal astigmatism. (Meta-analysis) PMID: 29422769
- The present study showed that PDGFRA amplification could be effectively targeted by pazopanib. PMID: 30060824
- KIT and PDGFRA mutations account for 85-90% of GISTs; subsequent genetic studies have led to the identification of mutation/epimutation of additional genes, including the succinate dehydrogenase (SDH) subunit A, B, C, and D genes. PMID: 29413424
- This study compared the efficacy of first-line therapy, doxorubicin (DOX), and TRAB in a platelet-derived growth factor receptor-alpha (PDGFRA)-amplified PLPS. METHODS: A fresh sample of PLPS tumor derived from a 68-year-old male patient diagnosed with a recurrent Pleomorphic liposarcoma was used in this study. PMID: 30126369
- The PDGFRA D842V mutant binds imatinib with lower affinity compared to the wild-type structure, exhibiting higher stability during interaction with other type I TKIs (like crenolanib). PMID: 29510530
- Altered SK3 channel expression observed in PDGFRalpha(+) cells in UPJ obstruction suggests that the impairment of SK3 activity across the UPJ may perturb upper urinary tract peristalsis in this urological condition. PMID: 28902181
- None of the 16 analyzable tumors showed mutations in PDGFRA. Thus, PDGFRA mutations likely do not play a significant role in the development of sporadic lipomas of the intestines. PMID: 26990750
- Whole transcriptome sequencing followed by pathway analysis indicated that STXBP4 is involved in functional gene networks that regulate cell growth, proliferation, cell death, and survival in cancer. Platelet-derived growth factor receptor alpha (PDGFRalpha) was a key downstream mediator of STXBP4 function. Consistent with this, shRNA-mediated STXBP4 and PDGFRA knockdown suppressed tumor growth in soft-agar and xenograft models. PMID: 28087642
- This study reports a unique case of an SDH-deficient GIST case with an activating PDGFRA mutation. Oncogenic mutations in GIST are generally mutually exclusive; however documented exceptions exist which may have diagnostic and therapeutic implications. PMID: 28768491
- Case Report: concurrent development of myeloproliferative hypereosinophilic syndrome and lymphomatoid papulosis associated with FIP1L1-PDGFRA gene fusion. PMID: 28374041
- The results of this study suggest that PDGFRalpha overexpression in HCC is a prognostic marker independent of adjacent non-tumor site liver fibrosis status. PMID: 28465473
- The balance of PDGFRalpha/PDGFRbeta signaling determines progenitor commitment to beige (PDGFRalpha) or white (PDGFRbeta) adipogenesis. PMID: 29158445
- This review provides an overview of primary cilia-mediated regulation of receptor tyrosine kinase (RTK- PDGFRa and PDGFRb) and transforming growth factor beta (TGF-beta) signaling. PMID: 27638178
- PDGFRA mutation, but not amplification, is associated with older age in pediatric high-grade glioma. PMID: 27582545
- This study demonstrates for the first time that PDGFR-alpha strongly inhibits endothelial and melanoma cells proliferation in a CXCL10/IP-10 dependent way, via miR-503 down-regulation. PMID: 27764787
- PDGFRalpha activation is an essential component that drives aggressiveness in papillary thyroid carcinoma cells. The signaling pathways are complex, involving not only the MAPK/Erk but also the PI3K/Akt and STAT3 pathways. PMID: 27845909
- This study provides a 19 A reconstruction for the cytomegalovirus gHgLgO trimer and shows that it binds with high affinity through the gO subunit to PDGFRalpha, which is expressed on fibroblasts but not on epithelial cells. PMID: 27573107
- Perivascular PDGFR-alpha and -beta were identified as independent markers predicting survival in metastatic colorectal cancer (mCRC). PMID: 27248825
- Data suggest that the platelet derived growth factor receptor alpha (PDGFRalpha)/Stat3 transcription factor/Rb1 protein regulatory axis might represent a potential therapeutic target for glioblastoma (GBM) treatment. PMID: 27344175
- Point mutations in the PDGFRa gene, which lead to amino acid residue changes activating the kinase of the receptor, occur in approximately 5% of Gastrointestinal Stroma Tumors. An activating deletion mutation of the PDGFRA gene has been described in a human Glioblastoma. PMID: 28940884
- FIP1L1/ PDGFRA associated chronic eosinophilic leukemia has an excellent long-term prognosis following imatinib therapy. PMID: 27120808
- Olaratumab had an acceptable adverse event profile in patients with gastrointestinal stromal tumor (GIST). While there was no apparent effect on PFS in patients without PDGFRa mutations, patients with PDGFRalpha-mutant GIST (all with D842V mutations) treated with olaratumab had longer disease control compared with historical data for this genotype. PMID: 28426120
- For hot spots in KIT and PDGFRA genes, 23 out of 146 KIT/PDGFRA wild-type cases carried mutations according to next-generation sequencing (NGS). PMID: 26848617
- In vitro activation of PDGFR-alpha leads to translational activation of LAMB1, which in turn induces an invasive and metastatic phenotype of hepatocellular carcinoma cells exhibiting K19 expression. PMID: 28783171
- PDGFRalpha levels are regulated by SMARCB1 expression, and assessment of clinical specimens documents the expression of both PDGFRalpha and FGFR1 in rhabdoid tumor patients. PMID: 27783942
- The downregulation of platelet-derived growth factor receptor-alpha expression may play a causative role in imatinib-induced thrombocytopenia, a common side effect, in the subset of chronic myeloid leukemia patients with platelet-derived growth factor receptor-alpha +68 GA ins/del, +68 GA del/del, and -909C/A genotypes. PMID: 29019285
- These findings support a distinct contribution of PDGFRalpha signaling to hepatic stellate cell proliferation and migration. PMID: 28734947
- Data indicate that co-inhibition of FGFR1 and HER2 or PDGFRalpha led to enhanced drug responses. PMID: 26549034
- High PDGFRA expression is associated with the pathogenesis of malignant peripheral nerve sheath tumor. PMID: 27477693
- The interaction between CSR1 and SF3A3 led to migration of SF3A3 from the nucleus to the cytoplasm. The cytoplasmic redistribution of SF3A3 significantly reduced the splicing efficiency of epidermal growth factor receptor and platelet-derived growth factor receptor. PMID: 27148859
- PDGFRa amplification in multiple skin lesions of undifferentiated pleomorphic sarcoma. PMID: 28105789
- In addition to representing a white adipose tissue (WAT) adipogenic niche, different PDGFRalpha(+) cell subsets modulate obesity-induced WAT fibrogenesis and are associated with loss of metabolic fitness. PMID: 28215843
- PDGFRA mutations were associated with gastrointestinal stromal tumors. PMID: 28098915
- The PDGFRA kinase domain structure reported in this study has potential to facilitate the development of new agents that can inhibit this kinase, including both its activating and drug-resistant mutations. PMID: 27349873
- BRAF mutations are rare events in KIT/PDGFRA wild-type gastrointestinal stromal tumors. PMID: 28159677
- There are two platelet-derived growth factor receptor (PDGFR) genes (PDGFRA and PDGFRB), and they reside on chromosome 4 and 5. PMID: 28267575
- Data did not detect any significant association with SNPs of APRIL, SPATA8, PDGFRA, and POLB with Systemic Lupus Erythematosus in the Chinese Han Population. PMID: 27569236
- Synchronous T lymphoblastic lymphoma and myeloid neoplasm with PDGFRA rearrangement. PMID: 28013529
- Stromal expression of PDGFRA increased with increasing histologic grade of breast phyllodes tumor. PDGFR stromal positivity was associated with shorter overall survival. PMID: 27881889
- Genome analysis of wild-type gastrointestinal stromal tumors for mutations should include the BRAF gene in patients with KIT and PDGFRA wild-type gastrointestinal stromal tumors. PMID: 27864688
- This study demonstrates that PDGFRalpha promotes dedifferentiation in PTC by decreasing TTF1 expression in the nucleus, which decreases iodide transport and thyroglobulin production in thyroid follicular cells. PMID: 27682510
- Increased PDGFRA expression is associated with thyroid papillary carcinoma. PMID: 26715280
- PDGFRA was a direct target of miR-34a in human pulmonary artery smooth muscle cells. PMID: 27302634
- The lack of PDGFRalpha(+)-cells in both the aganglionic and ganglionic Hirschsprung's disease bowel may contribute to the motility dysfunction. PMID: 27022215
- Ku80 and PDGFR-alpha might be effective predictive indicators for the prognosis of nasal type NK/T cell lymphoma. PMID: 26778387
- miR-140-5p acts as a tumor suppressor during ovarian carcinogenesis, inhibiting ovarian cancer growth partially by repressing PDGFRA expression. PMID: 26297547
- This study identified KIT and PDGFRA mutations in 21 out of 25 gastrointestinal stromal tumor samples from two referential national hospitals in Peru. PMID: 25659388
- A retrospective examination of correlations between clinical outcomes and KIT/PDGFRA mutational status in a subset of imatinib-resistant or -intolerant patients with stromal tumor participating in a worldwide, open-label treatment-use study was conducted. PMID: 26772734
- This study characterized metastatic exon 11 mutant gastrointestinal stromal tumors (GIST) genetic susceptibility genes beyond kit proto-oncogene protein (KIT)/PDGF alpha receptor (PDGFRalpha) genotype. PMID: 26544626
Database Links
Involvement In Disease
Gastrointestinal stromal tumor (GIST)
Protein Families
Protein kinase superfamily, Tyr protein kinase family, CSF-1/PDGF receptor subfamily
Subcellular Location
Cell membrane; Single-pass type I membrane protein. Cell projection, cilium. Golgi apparatus.
Tissue Specificity
Detected in platelets (at protein level). Widely expressed. Detected in brain, fibroblasts, smooth muscle, heart, and embryo. Expressed in primary and metastatic colon tumors and in normal colon tissue.
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