PDIA3 Antibody
Description
PDIA3 Overview
PDIA3, also termed ERp57 or GRP58, is a 57–60 kDa endoplasmic reticulum (ER)-resident chaperone with thiol oxidoreductase activity. It facilitates protein folding by catalyzing disulfide bond formation and rearrangement in glycoproteins . PDIA3 interacts with calnexin/calreticulin to regulate major histocompatibility complex (MHC) class I assembly and modulates STAT3 signaling, immune checkpoints (e.g., PD-1), and oxidative stress responses .
Clinical and Prognostic Significance in Cancer
PDIA3 overexpression correlates with tumor progression and poor prognosis across cancers. Key findings include:
Table 1: PDIA3 in Cancer Prognosis
Mechanistic Insights:
-
PDIA3 stabilizes STAT3 phosphorylation, amplifying pro-tumorigenic signaling .
-
In CRC, PDIA3 knockdown reduces invasiveness by 60% and proliferation by 45% .
Therapeutic Potential and Drug Targeting
PDIA3 is implicated in therapeutic resistance and immune evasion:
-
Immunotherapy Prediction: PDIA3 expression inversely correlates with anti-PD1/PD-L1 response in melanoma and urological cancers .
-
Inhibitor Screening: Connectivity Map (CMap) analysis identified PDIA3-linked compounds (e.g., HDAC inhibitors) as potential adjuvants .
-
Pulmonary Fibrosis: PDIA3 inhibition (e.g., LOC14) reduces fibrosis by suppressing SPP1 (osteopontin) in club cells .
Research Applications and Protocols
PDIA3 antibodies are utilized in diverse experimental setups:
-
Western Blot: Detects ~57 kDa bands in HeLa, HepG2, and glioma cell lysates .
-
Immunofluorescence: Localizes PDIA3 to the ER in U251 glioblastoma cells .
-
Single-Cell Analysis: PDIA3 is enriched in tumor-associated macrophages and malignant cells .
Technical Considerations
-
Antibody Validation: Prioritize antibodies verified via CRISPR/KO controls (e.g., PrecisionAb by Bio-Rad) .
-
Buffer Compatibility: Most antibodies are stable in PBS with 10% glycerol and 0.02% sodium azide .
Emerging Roles in Non-Cancer Pathologies
Product Specs
Product Science Overview
Mouse anti-human antibodies are secondary antibodies generated by immunizing mice with human antigens. These antibodies are used in various research and diagnostic applications to detect, sort, or purify human proteins. They are often conjugated with enzymes or fluorophores to facilitate detection in techniques such as ELISA, Western blotting, and immunohistochemistry .
Applications
- Detection: Used to identify human proteins in various assays.
- Sorting: Helps in the separation of human cells or proteins from a mixture.
- Purification: Facilitates the isolation of specific human proteins for further study .
Considerations
When using mouse anti-human antibodies, it is essential to be aware of the potential for the Human Anti-Mouse Antibody (HAMA) response. This response can occur when human patients develop antibodies against the mouse antibodies, leading to allergic reactions ranging from mild rashes to severe complications .
