Customize pdi-6 Antibody

Description

Target Profile of PDIA6 Antibody

PDIA6 antibodies specifically recognize the PDIA6 protein, a 48 kDa endoplasmic reticulum (ER)-resident oxidoreductase. Key features include:

Species Reactivity: Human, rat, mouse, and bovine .
Applications:
- Western blotting (WB)
- Immunocytochemistry/Immunofluorescence (ICC/IF)
- Flow cytometry (intracellular)
- Immunoprecipitation (IP) .
Epitope Specificity: Recombinant monoclonal antibodies (e.g., Abcam’s [EPR10132(B)]) target conserved regions, enabling cross-species reactivity .

Table 1: Validation Data for PDIA6 Antibodies

Application	Sample Type	Observed Band/Pattern
Western Blot	HL-1 cardiomyocytes	48 kDa band under hypoxia
Immunofluorescence	K-562 leukemia cells	ER-localized signal
Flow Cytometry	HT-1080 fibrosarcoma cells	Increased PDIA6 in permeabilized cells
ELISA	Mouse myocardial tissue	Elevated PDIA6 in infarcted hearts

Role in Cardiovascular Disease

Hypoxia Response: PDIA6 expression increases in hypoxic cardiomyocytes (HL-1 cells) and infarcted mouse heart tissue (LAD+ area), as confirmed by WB and immunohistochemistry .
Microbiome Interaction: Germ-free mice showed reduced PDIA6 upregulation post-myocardial infarction, linking gut microbiota to PDIA6-mediated UPR .

Cancer Biology

Oncogenic Role in NSCLC: PDIA6 is overexpressed in non-small cell lung cancer (NSCLC) and inhibits cisplatin-induced apoptosis and autophagy via MAP4K1/JNK/c-Jun signaling. Knockdown of PDIA6 sensitizes cancer cells to treatment .

Mechanism of Action

UPR Regulation: PDIA6 binds UPR sensors (e.g., ERN1, EIF2AK3) to attenuate IRE1 and PERK pathways, reducing ER stress-induced apoptosis .
Chaperone Activity: Prevents aggregation of misfolded proteins and supports platelet activation .

Critical Considerations for Experimental Use

Hypoxia-Specific Upregulation: PDIA6 expression peaks at 12 hours of hypoxia in cardiomyocytes but declines by 24 hours, necessitating precise timing in experiments .
Cross-Reactivity: Validate antibody specificity using knockout controls, as PDIA6 shares structural homology with other PDIs .

Product Specs

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Lead Time

Made-to-order (14-16 weeks)

Synonyms

pdi-6 antibody; tag-320 antibody; B0403.4 antibody; Protein disulfide-isomerase A6 homolog antibody; EC 5.3.4.1 antibody

Target Names

pdi-6

Uniprot No.

Q11067

Target Background

Function

PDI-6 Antibody may function as a chaperone that inhibits aggregation of misfolded proteins. It may also negatively regulate the unfolded protein response (UPR) by binding to UPR sensors.

Database Links

KEGG: cel:CELE_B0403.4

STRING: 6239.B0403.4

UniGene: Cel.6685

Protein Families

Protein disulfide isomerase family

Subcellular Location

Endoplasmic reticulum lumen.

Q&A

Here’s a structured FAQ collection for academic researchers working with PDI-6 Antibody, based on peer-reviewed studies and technical protocols:

Advanced Research Questions

How does PDIA6 influence Wnt secretion in inter-tissue signaling?

Mechanism: PDIA6 stabilizes Wnt ligands (e.g., EGL-20) via disulfide bond formation. Loss of PDIA6 leads to ER-associated degradation (ERAD) of misfolded Wnt proteins .
Experimental Design:
- Co-immunoprecipitation (IP) to confirm PDIA6-Wnt interactions .
- Use C. elegans models to study aging phenotypes linked to PDIA6 decline .

How to reconcile conflicting data on PDIA6’s role in autophagy?

Context-Dependent Analysis:

Study Context	PDIA6 Function	Key Finding
Pancreatic cancer models	Pro-autophagic	Silicasome nanoparticles enhance PDIA6-mediated immunogenic cell death
ER stress models	Anti-autophagic	PDIA6 stabilizes ER client proteins, reducing autophagic flux

Recommendation: Use tissue-specific KO models and monitor LC3B-II/I ratios under nutrient-deprived conditions .

Can PDIA6 antibody distinguish between redox states of the protein?

Approach:
- Treat cells with dithiothreitol (DTT) to reduce disulfide bonds.
- Compare antibody binding via redox SDS-PAGE (non-reducing vs. reducing conditions) .
Result: Reduced PDIA6 migrates faster (~48 kDa), while oxidized forms show higher MW .

Methodological Best Practices

Optimizing co-IP for PDIA6 interaction studies

Protocol:
- Use mild crosslinkers (e.g., DSP) to preserve transient interactions.
- Pre-clear lysates with protein A/G beads to reduce background .
- Include RNase treatment to exclude RNA-mediated artifacts (PDIA6 binds RNA in some contexts) .

Standardizing IF for PDIA6 in 3D organoid models

Key Steps:
- Permeabilize with 0.1% Triton X-100 for 10 min .
- Block with 5% BSA + 10% normal goat serum .
- Use Airyscan microscopy for ER sub-structure resolution .

Interdisciplinary Applications

Linking PDIA6 to mitochondrial unfolded protein response (UPR<sup>mt</sup>)

Strategy:
- CRISPR-Cas9 PDIA6 KO in intestinal cells; quantify mitochondrial ROS and HSP-60 levels .
- Correlate with lifespan assays in C. elegans (PDIA6 overexpression extends lifespan by 15–20%) .

Can PDIA6 antibodies detect pathological aggregates in neurodegeneration?

Validation Workflow:
- Stain post-mortem brain sections from Alzheimer’s patients.
- Compare with thioflavin-S (amyloid marker) co-localization .
- Use FRET-based probes to monitor PDIA6 activity in live neurons .

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pdi-6 Antibody