PDK Antibody
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Product Specs
Buffer
**Preservative:** 0.03% Proclin 300
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Made-to-order (14-16 weeks)
Synonyms
PDK antibody; PDHK antibody; YA5 antibody; At3g06483 antibody; F24P17.1 antibody; F5E6.19 antibody; [Pyruvate dehydrogenase antibody; acetyl-transferring)] kinase antibody; mitochondrial antibody; AtPDHK antibody; Pyruvate dehydrogenase kinase antibody; EC 2.7.11.2 antibody
Target Names
PDK
Uniprot No.
Target Background
Function
Pyruvate Dehydrogenase Kinase (PDK) is a serine protein kinase that regulates glucose metabolism by inhibiting the mitochondrial pyruvate dehydrogenase (PDH) complex (mtPDC). This inhibition is achieved through phosphorylation of the E1 alpha subunit of the PDH complex on Ser residues.
Gene References Into Functions
- The conserved Glu, Lys, and Asn residues within the N-box play a crucial role in coordinating Mg2+ within the PDK-MgATP-substrate ternary complex. This coordination is essential for the formation of the complex. PMID: 15629119
Database Links
Protein Families
PDK/BCKDK protein kinase family
Subcellular Location
Mitochondrion.
Tissue Specificity
Expressed constitutively and ubiquitously.
Q&A
Here’s a structured collection of FAQs for PDK1 antibody research, incorporating methodological guidance and data-driven insights from peer-reviewed sources:
Advanced Research Challenges
How to resolve contradictory PDK1 localization data between studies?
What humanization strategies preserve PDK1 antibody function while reducing immunogenicity?
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CDR grafting: Retain only 8-12 critical framework residues from murine origin .
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Affinity maturation: Use phage display to recover lost binding after humanization .
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Manufacturability testing: Screen for aggregation propensity via SEC-HPLC during reformatting .
How to quantify PDK1 antibody binding kinetics for mechanistic studies?
Technical Optimization
What IgG subclass optimizes PDK1 antibody performance in functional assays?
How to engineer bispecific PDK1 antibodies without compromising affinity?
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