PDX-1 antibody
Description
Overview of PDX-1 Antibodies
PDX-1 antibodies are immunological reagents designed to detect and quantify PDX-1, a homeodomain-containing transcription factor critical for:
These antibodies are available in monoclonal (e.g., clones EP139, F109-D12, B-11) and polyclonal forms, with reactivity across species (human, mouse, rat) .
Table 1: PDX-1 Antibody Clones and Properties
Research Applications
PDX-1 antibodies enable:
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Immunohistochemistry (IHC): Localizing PDX-1 in pancreatic tissues (e.g., β-cells, duodenum) .
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Flow Cytometry (FACS): Quantifying PDX-1 expression in INS-1 cells and stem cell-derived β-cells .
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Functional Studies: Investigating PDX-1 phosphorylation (e.g., Ser-61) and post-translational modifications .
PDX-1 as an Autoantigen in Type 1 Diabetes (T1D)
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Autoantibodies against PDX-1 are present in 50% of NOD mice and T1D patients, targeting C-terminal (rodents) and mid-region (humans) epitopes .
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PDX-1 autoreactivity correlates with β-cell destruction and impaired regeneration .
Therapeutic Potential
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Overexpression of PDX-1 promotes β-cell differentiation and insulin secretion .
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Gene therapy approaches using PDX-1 restore glucose homeostasis in diabetic models .
Post-Translational Regulation
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Phosphorylation at Ser-61 modulates PDX-1 nuclear localization and transcriptional activity .
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Negative feedback loops regulate PDX-1 levels to prevent β-cell dysfunction .
Ongoing Challenges
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Cross-Reactivity: Some clones (e.g., F6A11) lack reactivity with human PDX-1 .
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Standardization: Variability in epitope recognition (N-terminal vs. C-terminal) complicates data comparison .
PDX-1 antibodies remain indispensable for unraveling β-cell biology and advancing diabetes therapeutics. Recent studies highlight their utility in regenerative medicine, particularly in reprogramming non-β cells into insulin-producing cells . Future work should address antibody standardization and expand clinical validations.
Product Specs
Product Science Overview
The Pancreas Duodenum Homeobox-1 (PDX-1) protein, also known as insulin promoter factor 1 (IPF1), is a critical transcription factor involved in the regulation of insulin gene expression and pancreatic development. The polyclonal rabbit antibody against PDX-1 is widely used in research to study the protein’s role in various biological processes, including diabetes and pancreatic function.
PDX-1 is a homeodomain-containing transcription factor that plays a pivotal role in the development of the pancreas and the regulation of insulin gene transcription in pancreatic beta-cells . It is expressed in all cells during the early stages of development and is mainly restricted to the pancreas and duodenum in adults . PDX-1 is essential for the proper formation of the pancreas, and its absence can lead to pancreatic agenesis .
PDX-1 has been identified as a novel beta-cell-specific autoantigen for type 1 diabetes (T1D) . Autoantibodies against PDX-1 (PAAs) have been detected in non-obese diabetic (NOD) mice and human T1D patients . These autoantibodies are present before the onset of clinically overt diabetes, suggesting that PDX-1 plays a significant role in the autoimmune response leading to T1D .
The polyclonal rabbit antibody against PDX-1 is generated by immunizing rabbits with a peptide corresponding to a specific region of the PDX-1 protein. This antibody is used in various applications, including Western blotting, immunohistochemistry, and ELISA . It has been validated for reactivity with human, mouse, and rat samples .
- Western Blotting: The PDX-1 antibody is used to detect the presence of PDX-1 protein in cell lysates. It is typically used at a dilution of 1:500-1:1000 .
- Immunohistochemistry: This antibody is used to stain tissue sections to visualize the localization of PDX-1 in different tissues .
- ELISA: The antibody can be used in enzyme-linked immunosorbent assays to quantify the amount of PDX-1 protein in various samples .
