PEX19-2 Antibody

PEX19 Antibody Overview

PEX19 antibodies are immunological tools designed to detect and study the PEX19 protein, which functions as:

• A cytosolic chaperone for peroxisomal membrane proteins (PMPs) .

• An import receptor for PMPs, facilitating their insertion into peroxisomal membranes .

• A regulator of peroxisome biogenesis disorders (PBDs), including Zellweger syndrome .

No validated "PEX19-2" isoform or antibody is documented in UniProt, GeneCards, or peer-reviewed studies. Existing antibodies target canonical PEX19 and are critical for studying peroxisome dynamics and associated diseases.

Table 1: Validated PEX19 Antibodies

3.1. Role in Viral Replication

• PEX19 interacts with the influenza A virus M2 protein, inhibiting viral replication by reducing peroxisome abundance and disrupting ROS metabolism .

• Knockdown of PEX19 in A549 cells increased viral replication and oxidative stress .

3.2. Peroxisome Biogenesis and Disease

• PEX19 deficiency disrupts peroxisome assembly, leading to cytosolic catalase accumulation and Zellweger syndrome .

• Antibodies like ab137072 confirmed PEX19’s absence in CRISPR-edited PEX19⁻/⁻ cells via Western blot and immunofluorescence .

3.3. Neutral Lipid Metabolism

• PEX19 knockout cells (PEX19⁻/⁻) show defective lipid droplet turnover under catabolic conditions, independent of peroxisome function .

• Antibody 14713-1-AP detected PEX19 in lipid metabolic studies using human liver cancer tissue .

Table 2: Key Validation Experiments

Technical Considerations

• Band Discrepancies: Multiple antibodies report higher observed molecular weights (~35–40 kDa) than predicted (33 kDa), likely due to post-translational modifications .

• Cross-Reactivity: Antibody 14713-1-AP shows reactivity with Arabidopsis, suggesting conserved epitopes .

• Buffers: Antigen retrieval with TE buffer (pH 9.0) optimizes IHC detection .