phf23b Antibody

PHF23 Antibody Overview

PHF23 antibodies are immunological tools targeting the PHD finger protein 23, which regulates autophagy and cancer progression. These antibodies are primarily polyclonal, raised in rabbits, and validated for applications such as:

• Western blot (WB)

• Immunoprecipitation (IP)

• Immunohistochemistry (IHC)

• Immunofluorescence (IF)

Key characteristics include:

• Epitopes: Most target the N-terminal region (e.g., aa 1–403) or specific domains (e.g., aa 250–300) .

• Reactivity: Human, mouse, rat, cow, dog, pig, and rabbit .

• Validation: Confirmed via siRNA knockdown, peptide blocking, and functional assays .

Role in Autophagy Regulation

PHF23 inhibits autophagy by promoting ubiquitination and proteasomal degradation of LRSAM1, an E3 ligase critical for antibacterial autophagy :

Oncogenic Role in Non-Small Cell Lung Cancer (NSCLC)

PHF23 stabilizes ACTN4 (α-actinin-4) by blocking RNF38-mediated ubiquitination, activating ERK-c-Myc signaling:

Technical Considerations

• Buffer: Most antibodies are supplied in PBS with 0.09% sodium azide and 2% sucrose .

• Storage: Stable at -20°C; avoid freeze-thaw cycles .

• Controls: Recommended positive controls include HeLa, U2OS, and NSCLC cell lysates .