At2g43860 Antibody

Biological Function and Research Findings

• Role in Cell Wall Remodeling:
  At2g43860 is upregulated during ethylene-mediated cell wall loosening, particularly in tissues undergoing separation or expansion, such as the abscission zone (AZ) and germinating seeds .

  • In germinating tomato seeds, homologs of At2g43860 exhibit increased expression in endosperm cells, correlating with radicle emergence .

  • Ethylene treatment induces transcriptional activation of At2g43860 in AZ cells, suggesting its role in cell wall degradation during abscission .

• Developmental Regulation:
  Studies using microarray data identified At2g43860 as an auxin- and ethylene-responsive gene, with expression peaks during late stages of hormone signaling .

Applications of the At2g43860 Antibody

The antibody against At2g43860 is primarily used to:

1. Localize Protein Expression:

   • In situ hybridization and immunohistochemistry studies confirm tissue-specific expression in seed endosperm and AZ cells .

2. Quantify Protein Levels:

   • Western blotting detects dynamic changes in At2g43860 during ethylene-induced abscission or germination .

3. Functional Studies:

   • Knockout mutants or antibody-mediated inhibition of At2g43860 delay seed germination and reduce cell separation efficiency, validating its enzymatic role .

Table 1: Hormonal Regulation of At2g43860

Table 2: Tissue-Specific Expression

Research Implications

• Agricultural Biotechnology: Modulating At2g43860 activity could enhance seed germination rates or delay fruit abscission in crops .

• Disease Resistance: Pectin lyases like At2g43860 are targets for pathogens; understanding their regulation may improve plant immunity strategies .