PHKA1 Antibody, FITC conjugated

Antibody Platform

• Clone/Type: Monoclonal (e.g., EPR12118) or polyclonal (e.g., 24279-1-AP) rabbit IgG antibodies targeting PHKA1 .

• FITC Conjugation:

  • FITC (Fluorescein Isothiocyanate) is covalently attached to the antibody’s primary amines, creating a fluorescent probe.

  • Conjugation-ready versions (e.g., BSA/azide-free formats like ab249913) are optimized for labeling with fluorophores or enzymes .

Key Use Cases

1. Immunofluorescence (IF):

   • Localization of PHKA1 in muscle or liver cells.

   • Example: FITC-PHKA1 antibody could highlight PHKA1 distribution in glycogen-storing tissues.

2. Flow Cytometry:

   • Quantification of PHKA1 expression in cell populations.

   • Requires intracellular staining (Flow Cyt (Intra)) .

3. Western Blot (WB):

   • Detection of PHKA1 in lysates (e.g., HEK-293T, validated via knockout controls) .

Knockout Validation

• EPR12118 Antibody (ab176338):

  • Binds specifically to PHKA1 at 130 kDa in WT HEK-293T lysates.

  • No signal in PHKA1 knockout cells, confirming specificity .

Cross-Reactivity and Specificity

• Species: Human, mouse, rat .

• Isoforms: Detects PHKA1 isoforms in skeletal muscle (alpha-1) and liver (alpha-2) .

Challenges

• Hepatic Clearance: FITC-conjugated probes may exhibit rapid hepatic uptake, as seen in N-formyl peptide studies . This could influence in vivo applications.

• Conjugation Efficiency: Optimal FITC-to-antibody ratios must be balanced to avoid quenching or loss of antigen-binding capacity.