PHLDA2 Antibody
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Description
Introduction to PHLDA2 Antibody
The PHLDA2 Antibody (Catalog ID: A39576) is a rabbit polyclonal antibody designed for the detection of the human PHLDA2 protein, a critical regulator in cancer biology. This antibody is optimized for Western blotting (WB) and immunofluorescence (IF) applications, enabling researchers to study PHLDA2's role in signaling pathways, apoptosis, and tumor progression. Below is a detailed analysis of its specifications, applications, and research implications.
Research Context: PHLDA2 in Cancer Biology
PHLDA2 (pleckstrin homology-like domain family A member 2) is an imprinted gene located on chromosome 11p15.5. Its expression is tightly linked to oncogenic signaling pathways, including PI3K/AKT/mTOR . Key findings from PHLDA2 research include:
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Gastric Cancer: HGF stimulates PHLDA2 expression via the PI3K/AKT pathway, promoting cell proliferation and invasion while inhibiting apoptosis .
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Colorectal Cancer: PHLDA2 knockdown reduces tumor growth by activating autophagy and inhibiting epithelial-mesenchymal transition (EMT) .
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Lung Cancer: PHLDA2 is induced by EGFR/ErbB2 signaling and acts as a negative feedback regulator of AKT activation, modulating membrane lipid binding .
Applications of PHLDA2 Antibody in Research
The antibody's utility is evident in its compatibility with key techniques:
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Western Blotting:
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Immunofluorescence:
Key Research Findings Enabled by PHLDA2 Antibody
Product Specs
Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Lead Time
Typically, we can ship the products within 1-3 business days of receiving your order. Delivery time may vary depending on the purchasing method and location. Please consult your local distributors for specific delivery timeframes.
Synonyms
Beckwith Wiedemann syndrome chromosome region 1 candidate protein C antibody; Beckwith-Wiedemann syndrome chromosomal region 1 candidate gene C protein antibody; BRW 1C antibody; BRW1C antibody; BWR 1C antibody; BWR1C antibody; HLDA 2 antibody; HLDA2 antibody; Imprinted in placenta and liver antibody; Imprinted in placenta and liver protein antibody; IPL antibody; p17 Beckwith Wiedemann region 1C antibody; p17 BWR1C antibody; p17-Beckwith-Wiedemann region 1 C antibody; p17-BWR1C antibody; PHLA2_HUMAN antibody; PHLDA 2 antibody; phlda2 antibody; Pleckstrin homology like domain family A member 2 antibody; Pleckstrin homology-like domain family A member 2 antibody; TSSC 3 antibody; Tumor suppressing STF cDNA 3 protein antibody; Tumor suppressing subchromosomal transferable fragment candidate gene 3 protein antibody; Tumor suppressing subchromosomal transferable fragment cDNA 3 antibody; Tumor suppressing subtransferable candidate 3 antibody; Tumor supressing STF cDNA 3 antibody; Tumor-suppressing STF cDNA 3 protein antibody; Tumor-suppressing subchromosomal transferable fragment candidate gene 3 protein antibody
Target Names
PHLDA2
Uniprot No.
Target Background
Function
PHLDA2 plays a significant role in regulating placental growth. Its potential mechanism involves its PH domain, which competes with other PH domain-containing proteins, preventing them from binding to membrane lipids.
Gene References Into Functions
- PHLDA2 plays a critical role in the occurrence and development of pregnancy complications by promoting trophoblast apoptosis and suppressing cell invasion. PMID: 26935516
- TSSC3 has been identified as a prognostic marker in osteosarcoma. PMID: 27044808
- Downregulation of TSSC3 promotes epithelial-to-mesenchymal transition (EMT) in osteosarcoma cells by regulating EMT markers through a signaling pathway involving Snail, Wnt-beta-catenin/TCF, and GSK-3beta. PMID: 26845447
- Placental PHLDA2 expression was significantly elevated (2.3-fold) in pregnancies with reduced fetal movements leading to the delivery of a growth-restricted infant compared to those with a normal birth weight. PMID: 26944942
- PHLDA2 may contribute to the occurrence and development of preeclampsia by inhibiting the proliferation, migration, and invasion of trophoblasts. PMID: 26218012
- Gene expression levels of CDKN1C, H19, IGF2, KCNQ1, and PHLDA2 were evaluated using RT-PCR. PMID: 24986528
- Elevated expression of PHLDA2 in the placenta of growth-restricted pregnancies has been observed [review]. PMID: 24953163
- Research suggests that upregulated expression of pleckstrin homology-like domain family A member 2 (PHLDA2) in the placenta of monozygotic twins may be linked to the pathogenesis of singleton intrauterine growth restriction. PMID: 24703004
- Overexpression of TSSC3 suppressed osteosarcoma cell growth and increased apoptosis through caspase-3 upregulation, suggesting that TSSC3 may play a pro-apoptosis role in maintaining normal growth balance. PMID: 24268429
- TSSC3 inhibits osteosarcoma tumorigenicity by reducing stemness and promoting apoptosis in tumor-inducing cells. PMID: 22610481
- A luciferase reporter assay identified a 15 bp repeat sequence variant within the PHLDA2 promoter, significantly reducing PHLDA2 promoter efficiency. Maternal inheritance of this variant resulted in a significant increase in birth weight. PMID: 22444668
- Findings suggest that placental PHLDA2 may serve as a biomarker for suboptimal skeletal growth in pregnancies without overt fetal growth restriction. PMID: 22100507
- TSSC3 plays a potent tumor suppressor role in osteosarcoma, likely through the inhibition of growth and induction of apoptosis via the mitochondrial apoptosis pathway. PMID: 22021909
- PHLDA2 is a target gene of the BACH1 transcription factor according to ChIP-seq analysis in HEK 293 cells. PMID: 21555518
- Expression levels of the PHLDA2 gene were upregulated in the first trimester of pregnancy. PMID: 20484977
- The PHLDA2 gene exhibits imprinting, with preferential expression from the maternal allele in the placenta and liver. PMID: 9328465
- TSSC3 transcripts were not detected in human oocytes or preimplantation embryos. PMID: 15952111
- In vitro exposure of trophoblasts to hypoxia significantly reduced PHLDA2 expression. PMID: 16584773
- Association with low birth weight. PMID: 17180344
- Up-regulation of TSSC3 occurred in Dicer knockdown cells. PMID: 17303335
Database Links
Protein Families
PHLDA2 family
Subcellular Location
Cytoplasm. Membrane; Peripheral membrane protein.
Tissue Specificity
Expressed in placenta and adult prostate gland. In placenta, it is present in all cells of the villous cytotrophoblast. The protein is absent in cells from hydatidiform moles. Hydatidiform mole is a gestation characterized by abnormal development of both
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