pho-5 Antibody

PHO5 Protein Context

PHO5 is a Saccharomyces cerevisiae gene encoding a phosphate-repressible acid phosphatase critical for phosphate metabolism. Its promoter is a well-studied model for chromatin remodeling and transcriptional regulation . The protein is localized to the cell wall and vacuole, with expression tightly controlled by phosphate availability through transcription factors Pho4 and Pho2 .

Antibodies Mentioned in Search Results

While no PHO5-specific antibody is documented, several antibodies targeting phosphorylated proteins or regulatory factors are discussed:

Phospho-5-Lipoxygenase (Ser271) Antibody #3748

• Detects phosphorylation at Ser271 of 5-lipoxygenase (5-LO), an enzyme involved in leukotriene synthesis.

• Regulates 5-LO enzymatic activity in immune cells and cancer .

Phospho-Tau Antibodies

• Antibodies like AT8, PHF-1, and TG-3 are validated for specificity to phosphorylated tau epitopes (e.g., Ser202/Thr205, Ser396/Ser404) .

• Non-specific binding observed in antibodies like AT270 highlights challenges in phospho-specific antibody validation .

PHO5 Regulatory Mechanisms

PHO5 expression involves:

• Chromatin remodeling: NuA4 histone acetyltransferase recruitment by Pho2 under repressive conditions primes the promoter for activation .

• Antisense transcription: Non-coding antisense RNA represses PHO5 by promoting Rpd3-dependent histone deacetylation and delaying chromatin remodeling .

• Cell cycle coordination: Mcm1 and Fkh proteins collaborate with Pho4/Pho2 to drive mitotic induction .

Experimental Tools for PHO5 Studies

• Genetic screens: High-throughput assays using yeast deletion collections identify mutants affecting PHO5 expression kinetics .

• CRISPRi: Targeted repression of antisense transcription accelerates PHO5 induction, demonstrating direct regulatory interplay .

Table 1: PHO5 Regulatory Factors

Table 2: Antibody Specificity Metrics (from )