Phospho-ANAPC1 (S688) Antibody
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Description
Recommended Dilutions
| Application | Boster Bio | Cusabio |
|---|---|---|
| WB | 1:500–1:1000 | Not specified |
| IHC | Not validated | 1:100–1:300 |
| IF | Not validated | 1:200–1:1000 |
| ELISA | Not validated | 1:10,000 |
Both vendors validate specificity using positive/negative controls across multiple platforms . Boster Bio guarantees performance in WB , while Cusabio emphasizes IHC and IF applications .
Biological Significance of ANAPC1
ANAPC1 (Gene ID: 64682 ) is a core subunit of the APC/C, an E3 ubiquitin ligase essential for cell cycle progression. Key functions include:
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Metaphase-to-Anaphase Transition: Mediates ubiquitination of securin and cyclin B, enabling chromatid separation .
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Checkpoint Regulation: Integrates signals from the spindle assembly checkpoint to ensure genomic stability .
Phosphorylation at S688 is hypothesized to modulate APC/C activity during mitotic exit, though mechanistic details remain under investigation .
Research Considerations
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Limitations: No validation images or peer-reviewed publications are currently available for this antibody .
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Controls: Users must include phosphorylated and non-phosphorylated ANAPC1 samples to confirm specificity.
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Cross-Reactivity: No data exist for non-human species; applications are restricted to human samples .
Future Directions
Further studies are needed to:
Product Specs
Buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA, and 0.02% sodium azide.
Form
Liquid
Lead Time
Typically, we can dispatch products within 1-3 business days after receiving your orders. Delivery times may vary depending on the purchasing method or location. For specific delivery times, please consult your local distributors.
Synonyms
ANAPC 1 antibody; Anapc1 antibody; Anaphase promoting complex subunit 1 antibody; anaphase-promoting complex 1 (meiotic checkpoint regulator) antibody; Anaphase-promoting complex subunit 1 antibody; Apc 1 antibody; APC1 antibody; APC1_HUMAN antibody; Cyclosome subunit 1 antibody; MCPR antibody; Meiotic checkpoint regulator antibody; Mitotic checkpoint regulator antibody; Protein Tsg 24 antibody; Protein Tsg24 antibody; Testis-specific gene 24 protein antibody; TSG 24 antibody; TSG24 antibody
Target Names
Uniprot No.
Target Background
Function
Phospho-ANAPC1 (S688) Antibody is a component of the anaphase-promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that governs progression through mitosis and the G1 phase of the cell cycle. The APC/C complex functions by mediating the ubiquitination and subsequent degradation of target proteins. Primarily, it facilitates the formation of 'Lys-11'-linked polyubiquitin chains, and to a lesser extent, 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
Gene References Into Functions
- Research indicates that FZR1 (Fzr), a cofactor of the multi-subunit E3 ligase complex anaphase-promoting complex/cyclosome (APC/C), represents a therapeutic target in myeloma. PMID: 27655696
- In the Alzheimer's disease (AD) mouse model APP/PS1, reduced cdh1 levels were observed in pyramidal neurons in CA1 compared to age-matched wildtype mice. This review provides a comprehensive list of APC/C substrates involved in the nervous system and discusses their functions. It also summarizes recent studies demonstrating neurobiological effects in cdh1 knockout mouse models. PMID: 28505105
- The cryo-EM structure of an APC/C-Cdh1 complex with Apc1(WD40) deleted revealed that the mutant APC/C is locked in an inactive conformation where the UbcH10-binding site of the catalytic module is inaccessible. Additionally, an EM density for Apc15 is not visible. PMID: 27601667
- The ABBA-KEN-ABBA amino acid motif cassette holds the Mitotic Checkpoint Complex (MCC) onto the Anaphase-Promoting Complex-Cyclosome (APC/C) by binding the two Cdc20 molecules in the MCC-APC/C complex. PMID: 27939943
- Findings suggest a tumor suppressor role for APC/C(Cdh1) in melanocytes, and targeting PAX3 might be a strategy for treating melanoma. PMID: 26329581
- Human Cytomegalovirus UL21a, but not UL97, can disrupt APC/C function, leading to the accumulation of substrates and demonstrating that UL21a is both necessary and sufficient to induce the degradation of APC1, in addition to the previously reported APC4 and APC5. PMID: 25903336
- Data indicate that Rad17 turnover is regulated through the Cdh1/anaphase-promoting complex pathway in breast cancer cells. PMID: 23637229
- EYA1 is efficiently degraded during mitotic exit in an ANAPC1-dependent manner, and these two proteins physically interact. PMID: 23263983
- Single nucleotide polymorphisms of ANAPC1 were associated with nicotine dependence. PMID: 22377092
- Studies indicate that the APC/C is tightly regulated by its co-activators and by the spindle assembly checkpoint. PMID: 21633387
- The ability of Emi1 to inhibit APC/C is negatively regulated by CDKs. PMID: 21454540
- Data demonstrate that PFKFB3 is essential for cell division and that it is regulated by APC/C-Cdh1 and SKP1-CUL1-F (SCF)-beta-TrCP. PMID: 21402913
- Studies indicate that APC/C(Cdh1) is required to maintain genomic stability. PMID: 19826416
- Cdh1 may function as a component in tumor suppression via proteolysis of Skp2 in colorectal tumorigenesis and may serve as a prognostic marker in colon cancer patients. PMID: 18535175
- ANAPC1 methylation was likely not implicated in gastric carcinogenesis. PMID: 18622497
Database Links
Protein Families
APC1 family
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