Phospho-CDC25C (T48) Antibody

• Cells lacking ARID1A exhibit increased AURKA transcription, leading to persistent activation of CDC25C, a key protein for G2/M transition and mitotic entry. PMID: 30097580
• FHL1 enhances inhibitory CDC25 phosphorylation by forming a complex with CHK2 and CDC25, and sequesters CDC25 in the cytoplasm by forming another complex with 14-3-3 and CDC25, resulting in increased radioresistance in cancer cells. PMID: 28094252
• CDC25A plays a novel role in regulating the malignant behavior of glioma stem cells as part of the Linc00152/miR-103a-3p/FEZF1/CDC25A axis. PMID: 28651608
• Overexpression of CDK1 and CDC25A may contribute to the pathogenesis of the NFPA. PMID: 28004354
• Mdm2 overexpression and Cdc25C downregulation delay cell cycle progression through the G2/M phase. PMID: 28806397
• Xanthatin acts as a DNA-damaging agent in non-small cell lung carcinomas by activating Chk1-mediated DDR and lysosome-mediated degradation of Cdc25C. PMID: 29074359
• Myelodysplastic syndrome -related P95 point mutants of SRSF2 lead to alternative splicing of CDC25C in a manner independent of the DNA damage response. PMID: 27552991
• This review aims to illuminate the role of four different phosphatases (PTEN, PP2A, CDC25 and DUSP1) in five different solid tumors (breast cancer, lung cancer, pancreatic cancer, prostate cancer and ovarian cancer), to better understand the most frequent and aggressive primary cancer of the central nervous system, glioblastoma. PMID: 28801478
• Data show that TRIB2-mediated degradation of CDC25C is associated with lysine-48-linked CDC25C polyubiquitination driven by the TRIB2 kinase-like domain. PMID: 27563873
• High expression of pCHK2-Thr68 was associated with decreased patient survival (p = 0.001), but was not an independent prognostic factor. Our results suggest that pCHK2-Thr68 and pCDC25C-Ser216 play significant roles in breast cancer and may be potential treatment targets PMID: 27801830
• The biology of CDC25 activation/deactivation by kinases/phosphatases maintains the level of CDK-cyclin activities and thus genomic stability PMID: 27038604
• The knockdown of CDC25C can reduce both the radiotherapy sensitivity and the proliferation activity of EC9706 cells. PMID: 27188256
• Results identify CDC25C as a downstream target of the mutated tyrosine kinase FLT3-ITD affecting cell-cycle regulation in a model of AML PMID: 27919943
• Suggest that the p53-p21-DREAM-CDE/CHR pathway regulates p53-dependent repression of Survivin, CDC25C, and PLK1 in HCT116 cells. PMID: 26595675
• These miR-142-3p functioned as a tumor suppressor by targeting CDC25C. PMID: 26805039
• Cdc25C negatively regulates proapoptotic ASK1 in a cell cycle-dependent manner and may play a role in G2/M checkpoint-mediated apoptosis. PMID: 25633196
• Recurrent CDC25C mutations drive malignant transformation in familial platelet disorder to acute myelogenous leukaemia. PMID: 25159113
• We conclude that inhibition of KIF22 suppresses cancer cell proliferation by delaying mitotic exit through the transcriptional upregulation of CDC25C. PMID: 24626146
• These findings indicate that DHM inhibits the growth of hepatocellular carcinoma (HCC) cells via G2/M phase cell cycle arrest through the Chk1/Chk2/Cdc25C pathway PMID: 24002546
• Purification and biochemical analysis of catalytically active human cdc25C dual specificity phosphatase PMID: 23567337
• The protein plays a role in regulating PCa cell growth, and androgen treatments, but not EGF, greatly increase Cdc25C protein levels in AS PCa cells, which is in part by decreasing its degradation. PMID: 23637932
• Dose-dependent Cdc25c phosphatase acts as an early G2-phase checkpoint, thus indicating mechanistic importance in the low-dose hyper-radiosensitivity and induced radioresistance transition PMID: 22843362
• Data suggest that the maintenance of CDC25 activity does not fully rely on the thioredoxin reductase system in breast cancer cells, even in the presence of major oxidative stress PMID: 22360685
• Cloning and functional analysis of Cdc25C PMID: 22394631
• MMEQ induced G2/M arrest through the promotion of cdc25c in TSGH8301 cells. PMID: 22021033
• Inhibition of CK2 activity by three different inhibitors led to a down-regulation of the level of cdc25C. PMID: 21750987
• Two additional sites other than Ser216 in the widely studied cell division cycle (Cdc) protein 25C, whose function depends on 14-3-3 binding, were identified. PMID: 21189416
• The results show for the first time that in human mitosis, distinct phospho-isoforms of cdc25C exist with different localizations and interacting partners. PMID: 20668692
• ATM and Chk1/2 mediated phosphorylation of cdc25c plays a major role in cell cycle arrest induced by pectenotoxin2. PMID: 20514472
• CDC25C and phospho-CDC25C (Ser216) play a crucial role in the pathogenesis and/or progression of vulvar squamous cell carcinomas. PMID: 20500813
• Adventitious arsenate reductase activity of the catalytic domain of the human and Cdc25C phosphatases PMID: 20025242
• Analysis of cell cycle profile and cell cycle regulatory proteins indicated that arsenite arrested cell cycle at G(2)/M phase, partially through induction of cell division cycle 25 (Cdc25) isoform C (Cdc25C) degradation via ubiquitin-proteasome pathways PMID: 11842186
• Results suggest that Plk1 phosphorylates Cdc25C on Ser198 and regulates nuclear translocation of Cdc25C during prophase PMID: 11897663
• Role of degradation by oxidative stress in induction of cell cycle arrest PMID: 11925443
• Human CDC25B and CDC25C differ in their ability to restore a functional checkpoint response after gene replacement in fission yeast PMID: 12099692
• Ca2+ promotes erythrocyte band 3 tyrosine phosphorylation via dissociation of phosphotyrosine phosphatase from band 3. PMID: 12175337
• Phosphorylation by Chk2 PMID: 12386164
• CDC25C is phosphorylated on Ser 214 during mitosis, which prevents phosphorylation of Ser 216. HeLa cells depleted of endogenous CDC25C, when treated with exogenous CDC25C, showed a substantial delay in mitotic entry. PMID: 12766774
• Cdc25C plays a role not only at the G2/M transition but also in the modulation of DNA replication PMID: 12857880
• CDC25C translocation to the cell nucleus upon entry into mitosis is coordinated by Plk3 PMID: 14968113
• Binding to VPR protein in human cell lines correlates with G2 arrest PMID: 14972559
• Downregulation of Cdc25C is mediated by p53 via two independent mechanisms, one involving direct binding to the cdc25C promoter PMID: 15574328
• Vpr promotes cell cycle arrest at the G(2)/M phase by facilitating association of 14-3-3 and Cdc25C PMID: 15708996
• Vitamin C transiently arrests cancer cell cycle progression in S phase and G2/M boundary by modulating the kinetics of activation of CDC25C. PMID: 15887239
• Data suggests that CDC25C might play a significant role in prostate cancer progression and could be used to monitor and predict the aggressiveness of this disease. PMID: 16000564
• Data suggest that Pim-1 activates Cdc25C by direct phosphorylation and can thereby assume the function of a positive cell cycle regulator at the G2/M transition. PMID: 16356754
• Crystallization experiments of PLK1 protein in complex with an unphosphorylated and a phosphorylated target peptide from Cdc25C yield crystals suitable for X-ray diffraction analysis. PMID: 16582488
• These results demonstrate that the MAPK ERK signaling pathway contributes to the p53-independent antiproliferative functions of p14ARF. Furthermore, they identify a new mechanism by which phosphorylation at serine 216 participates in Cdc25C inactivation. PMID: 16582626
• Phosphorylation of cdc25c can be used to test whether a pharmacologic inhibitor of Plk1 would exert the same cellular effects as interference with Plk1 at an mRNA level. PMID: 16648550
• Chk1-mediated phosphorylation of Cdc25C plays a major role in response to LOR-mediated G(2)/M arrest. Although the Chk1-mediated cell growth arrest in a tumor cell line. PMID: 16649252

HGNC: 1727

OMIM: 157680

KEGG: hsa:995

STRING: 9606.ENSP00000321656

UniGene: Hs.656