Phospho-MAP3K9/MAP3K10 (T312/266) Antibody

MAP3K9 (MLK1)

• Function: Activates JNK signaling through phosphorylation of MKK4/SEK1, implicated in nerve growth factor (NGF)-induced apoptosis .

• Gene ID: 4293 (UniProt: P80192) .

• Domain Structure: Contains SH3, kinase, and leucine zipper domains for protein-protein interactions .

MAP3K10 (MLK2)

• Function: Regulates stress-activated pathways via JNK and p38 MAPK cascades, with roles in inflammation and cell differentiation .

• Gene ID: 4294 (UniProt: Q02779) .

Research Applications

This antibody has been validated in:

• Immunohistochemistry (IHC): Localizes activated MAP3K9/10 in formalin-fixed, paraffin-embedded tissues .

• Western Blot: Detects phosphorylated MLK1/2 in lysates from stressed neuronal cells .

• Mechanistic Studies: Used to study JNK pathway activation in:

  • Neurodegeneration models

  • Cancer cell apoptosis

  • Inflammatory signaling

Key Research Findings

Recent studies using this antibody have revealed:

Technical Considerations

• Specificity: Recognizes phosphorylated T312 (MAP3K9) and T266 (MAP3K10) but not unphosphorylated forms or other MAP3K isoforms .

• Cross-Reactivity: None observed with MAP3K11 or other MLK family members .

• Validation: Verified using siRNA-mediated kinase knockdown controls in peer-reviewed studies .