Phospho-MAPK9/MAPK10 (Thr183) Antibody
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Lead Time
Typically, we can ship your orders within 1-3 business days of receipt. Delivery times may vary depending on the purchase method or location. Please contact your local distributor for specific delivery time information.
Synonyms
c Jun kinase 2 antibody; C Jun N terminal kinase 2 antibody; c-Jun N-terminal kinase 2 antibody; JNK 55 antibody; JNK-55 antibody; JNK2 alpha antibody; JNK2 antibody; JNK2 beta antibody; JNK2A antibody; JNK2alpha antibody; JNK2B antibody; JNK2BETA antibody; Jun kinase antibody; MAP kinase 9 antibody; MAPK 9 antibody; Mapk9 antibody; Mitogen activated protein kinase 9 antibody; Mitogen-activated protein kinase 9 antibody; MK09_HUMAN antibody; P54a antibody; p54aSAPK antibody; PRKM9 antibody; Protein kinase, mitogen-activated, 9 antibody; SAPK alpha antibody; SAPK antibody; SAPK1a antibody; Stress activated protein kinase 1a antibody; Stress-activated protein kinase JNK2 antibody
Target Names
MAPK9/MAPK10
Uniprot No.
Target Background
Function
Serine/threonine-protein kinase involved in various cellular processes such as proliferation, differentiation, migration, transformation, and programmed cell death. Extracellular stimuli, such as proinflammatory cytokines or physical stress, activate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases, MAP2K4/MKK4 and MAP2K7/MKK7, phosphorylate and activate MAPK9/JNK2. Subsequently, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1, such as JUN and ATF2, thereby regulating AP-1 transcriptional activity. In response to oxidative or ribotoxic stresses, MAPK9/JNK2 inhibits rRNA synthesis by phosphorylating and inactivating the RNA polymerase 1-specific transcription initiation factor RRN3. It promotes stressed cell apoptosis by phosphorylating key regulatory factors, including TP53 and YAP1. In T-cells, MAPK8 and MAPK9 are essential for the polarized differentiation of T-helper cells into Th1 cells. Following T-cell receptor (TCR) stimulation, MAPK9/JNK2 is activated by CARMA1, BCL10, MAP2K7, and MAP3K7/TAK1 to regulate JUN protein levels. MAPK9/JNK2 plays a crucial role in osmotic stress-induced epithelial tight-junction disruption. Upon activation, it promotes beta-catenin/CTNNB1 degradation and inhibits the canonical Wnt signaling pathway. MAPK9/JNK2 also participates in neurite growth in spiral ganglion neurons. It phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer, playing a role in the regulation of the circadian clock. Furthermore, MAPK9/JNK2 phosphorylates POU5F1, resulting in the inhibition of POU5F1's transcriptional activity and enhancing its proteosomal degradation. MAPK9 isoforms exhibit distinct binding patterns: alpha-1 and alpha-2 preferentially bind to JUN, while beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which occurs at approximately the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it.
Gene References Into Functions
- Collectively, silencing of H4R inhibited H4R-mediated Mast cell functions and SAPK/JNK phosphorylation. Furthermore, H4R activation utilized the SAPK/JNK signaling pathway for IL-1beta release in HMC-1 cells. PMID: 29863427
- RhoGDIbeta overexpression led to downregulation of miR-200c, while miR-200c directly targeted the 3'-UTR of jnk2mRNA, attenuating JNK2 protein translation. This resulted in the attenuation of Sp1mRNA and protein expression, ultimately inhibiting Sp1-dependent MMP-2 transcription. PMID: 28846829
- The MAP kinase JNK2 mediates cigarette smoke-induced tissue factor activation, arterial thrombosis, and reactive oxygen species production. PMID: 27761579
- We observed up-regulation of p-JNK2 in AUC and its early down-regulation in UC-CRC and CRC carcinogenesis. PMID: 28383667
- JNK2 was identified as a novel direct target of miR-20a-5p. PMID: 27494776
- Interleukin-1 acts through the JNK-2 signaling pathway to induce aggrecan degradation by human chondrocytes. PMID: 25776267
- miR200c attenuates P-gp-mediated MDR and metastasis by targeting the JNK2/c-Jun signaling pathway in colorectal cancer. PMID: 25205654
- JNK2 activates pro-survival autophagy and inhibits palmitic acid lipotoxicity in hepatocytes. PMID: 24608675
- Our findings demonstrate that AKT/mTOR and JNK2 signaling pathways are required for chrysotile asbestos-induced autophagy. PMID: 24735948
- These results indicate that JNK2 is essential for the maintenance of normal intestinal epithelial homeostasis and maturation under biological conditions by differentially modulating HuR and CUGBP1. PMID: 24740539
- After overexpression and knockdown of XIAP, the AKT, pAKT, JNK2, and GSK3beta levels were determined in periodontal ligament cells. PMID: 24802394
- This study demonstrates that cyclic stretch disrupts tight junctions and adherens junctions through a JNK2, c-Src, and MLCK-dependent mechanism. PMID: 24722904
- Poly(ADP-ribose) polymerase family member 14 (PARP14) is a novel effector of the JNK2-dependent pro-survival signal in multiple myeloma. PMID: 23045269
- SREBP-1c is a novel insulin/JNK2-regulated gene, and the JNK2/SREBP-1c pathway mediates insulin-induced fatty acid synthesis, potentially leading to enlargement of LDs in human adipocytes. PMID: 23515281
- JNK2 is activated during endoplasmic reticulum stress and promotes cell survival. PMID: 23171849
- Data indicate that WDR62 dimerization is required for JNK2 and MKK7beta1 recruitment. PMID: 23341463
- We observed that the phosphorylated expression of JNK2 was upregulated in the skin of patients with hypospadias compared to the normal prepuce at both mRNA and protein levels. PMID: 23273084
- The effect of APRIL is mediated through BCMA, which does not activate the classical NF-kappaB pathway. Instead, it induces a novel signaling pathway involving JNK2 phosphorylation, FOXO3A activation, and GADD45 transcription. PMID: 23071284
- JNK2 regulated nucleolin expression, which might in turn stabilize hif-1alpha mRNA. PMID: 22910906
- SEPW1 silencing increases MKK4, which activates p38gamma, p38delta, and JNK2 to phosphorylate p53 on Ser-33, causing a transient G(1) arrest. PMID: 22730327
- Ulinastatin inhibits the proliferation of human breast cancer cells and the growth of xenografted tumors. This mechanism might be related to the decrease in signal transduction of JNk-2 and NF-kappaB. PMID: 22217202
- Up-regulation of hepatic MRP3/ABCC3 expression in human obstructive cholestasis is likely triggered by TNFalpha, mediated by the activation of JNK/SAPK and SP1. PMID: 22105759
- The JNK/SAPK signal pathway plays a significant role in regulating bFGF-mediated angiogenesis in HUVECs. PMID: 21572099
- WDR62 signaling association with JNK2 requires both the JNK2 Cys-Asp (CD) and Glu-Asp (ED) domains. PMID: 21749326
- A critical role for JNK2 and EPS8 in receptor tyrosine kinase signaling and trafficking to convey distinctly different effects on cell migration. PMID: 21357683
- JNK2alpha3 and JNK2beta3 showed different levels of substrate phosphorylation, although both could promote the proliferation of 293T cells. PMID: 21110917
- These data demonstrate, for the first time, that JNK2a can regulate the transcriptional activity of STAT3 by phosphorylating the Ser727 residue, thereby regulating the expression of oncogenic genes, such as c-Myc. PMID: 20871632
- The role of JNK2 in the mechanism of osmotic stress-induced tight junction disruption in the intestinal epithelium in 3 species is described. PMID: 20595622
- JNK2 collaborates with other oncogenes, such as Ras, at multiple molecular levels to promote tumorigenesis. PMID: 20354187
- Our results indicate that the activation of phosphorylated c-Jun NH2-terminal kinase may play a role in the carcinogenesis of basal-like and triple-negative breast carcinoma. PMID: 19913278
- Protein kinase D complexes with C-Jun N-terminal kinase via activation loop phosphorylation and phosphorylates the C-Jun N-terminus. PMID: 11948398
- Involves TRAF4 in oxidative activation. PMID: 12023963
- A biochemical pathway exists wherein fluid shear activates cyclooxygenase-2 via a c-Jun N-terminal kinase2/c-Jun-dependent pathway, which in turn elicits downstream prostaglandin EP2 and EP3a1 receptor mRNA synthesis. PMID: 12743126
- JNK2 functions in maintaining the genomic stability of mammalian cells by signaling that is independent of cyclin-dependent kinase 1/cyclin B1 down-regulation. PMID: 15262983
- Analysis of the regions required for JNK2alpha2 autophosphorylation. PMID: 15637069
- JIP1 and JIP3 exhibit cross-talk that leads to the regulation of the ASK1-SEK1-JNK signal during glucose deprivation. Cross-talk between JIP3 and JIP1 is mediated through SEK1-JNK2 and Akt1. PMID: 15911620
- Results showed that aberrant p-JNK1/2 expression and the co-expressed p-JNK1/2 and p-p38 in breast tissues may play a role in the carcinogenesis of breast IDC. PMID: 16381010
- Results indicated that certain regulation involved in c-myc, c-fos, and c-jun was present in the apoptosis, and the c-Myc dependent-on and Jun N-terminal kinase (JNK) pathway also play roles. PMID: 16552729
- We defined the kinetic mechanism for the active form of JNK2alpha2. PMID: 17397142
- Results indicated that purified JNK2 from transfected nonstressed 293T cells is a mixture of the mono-sites pThr183 and pTyr185 of its activation loop and of pThr386 along its unique C-terminal region. PMID: 17637567
- Neutrophils from transgenic JNK2 knockout mice (which are devoid of all JNK isoforms) demonstrate a requirement for JNK2 in Toxoplasma gondii infection-induced interleukin-12p40 and CCL2/MCP-1 production. PMID: 17785791
- These findings are consistent with JNK2 signaling playing a protective role in acetaminophen-induced liver injury. PMID: 18586006
- JNK2 is a player in triglyceride (TG) homeostasis and lipid droplet metabolism, specifically in the regulation of lipolysis. PMID: 18612143
- Results suggest that the MAP kinase insert of JNK2 plays a role in the regulation of JNK2 activation, possibly by interacting with intracellular binding partners. PMID: 18801372
- JNK2 is linked, directly or indirectly, with SIRT1 protein stability, and this function is coupled with SIRT1 phosphorylation at serine 27. PMID: 18838864
- JNK2 alpha2 activity is dependent on a unique mechanism of MAPK activation. PMID: 18940813
- Detection of the mutation could characterize a subset of ET patients with a distinct phenotype, despite its clinical significance being still undetermined. PMID: 19093167
- Knock-down of JNK2 unexpectedly increased the intensity of differentiation in human myeloid leukemia cells. PMID: 19339050
- Both cell protective (cell cycle progression) and destructive mechanisms (apoptosis) are simultaneously controlled by a single JNK isoform within the same cell system, even under the influence of one stimulus. PMID: 19385049
- NAG7 plays a potential role in promoting nasopharyngeal carcinoma invasion by regulation of ERalpha and the H-ras/p-c-Raf and JNK2/AP-1/MMP1 signaling pathways. PMID: 19591174
Database Links
Protein Families
Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily
Subcellular Location
Cytoplasm. Nucleus.
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
