Phospho-NCF1 (S359) Antibody

• IL-27 enhances reactive oxygen species (ROS) generation from monocyte-derived macrophages and dendritic cells by inducing p47(phox). PMID: 28240310
• p47phox, but not p67phox or p40phox, binds to and activates Nrf2, thus enhancing Nrf2's function in suppressing inflammation. PMID: 28939422
• S-glutathionylation of p47phox plays a critical role in sustained ROS generation by human neutrophils. PMID: 29195919
• Patients with hereditary p47phox deficiency exhibit reduced platelet activation, suggesting a role for this Nox cytosolic subunit in platelet activation. PMID: 27723093
• Decreased and increased copy numbers of NCF1 predispose to and protect against SLE, respectively. PMID: 28135245
• Lysophosphatidylcholines prime polymorphonuclear neutrophils through Hck-dependent activation of PKCdelta, which stimulates PKCgamma, resulting in translocation of phosphorylated p47(phox). PMID: 27531930
• There was an increased frequency of the NCF1-339 T allele in patients with systemic lupus erythematosus. The NCF1-339 T allele reduced extracellular ROS production in neutrophils and led to increased expression of type 1 interferon-regulated genes. PMID: 28606963
• Skeletal muscle protein expression of the NADPH oxidase subunits p22(phox), p47(phox), and p67(phox) was elevated in obese subjects compared to lean subjects, where p22(phox) and p67(phox) expression was attenuated by exercise training in obese subjects. PMID: 27765769
• A novel role for Spns2 and S1P1&2 in the activation of p47(phox) and production of reactive oxygen species is involved in hyperoxia-mediated lung injury. PMID: 27343196
• This study provides evidence for a novel PKC-zeta to p47phox interaction that is required for cell transformation from blebbishields and ROS production in cancer cells. PMID: 27040869
• Overexpression of p47phox is associated with increased migration/metastasis rate in melanoma. PMID: 26760964
• A rare mutation in NCF1 encoding p47phox of the leukocyte NADPH oxidase causes a lack of superoxide generation, leading to chronic granulomatous disease. This mutation was recently (1200-2300 years ago) introduced into the Kavkazi Jewish population. PMID: 26460255
• Data show that diphenylene iodonium (DPI) and apocynin can reduce hyperoxia-induced reactive oxygen species (ROS) production by decreasing the translocation and level of NADPH Oxidase p47phox. PMID: 26728380
• Increased levels of gp91phox, p47phox, and p22phox likely account for the interferon-gamma mediated enhancement of dimethyl sulfoxide-induced Nox2 activity. PMID: 26317224
• This research identifies p47phox-dependent NADPH oxidase activity as a critical component of Angpt-1-mediated endothelial barrier defense against classic inflammatory permeability factors. PMID: 25761062
• DCLRE1C and NCF1 mutations have been found by whole-genome sequencing to cause primary immunodeficiency in unrelated patients. PMID: 25981738
• TLR8, but not TLR7, is involved in priming of human neutrophil reactive oxygen species production by inducing the phosphorylation of p47phox and p38 MAPK. PMID: 25877926
• Reduced carotid but not coronary artery atherosclerosis in patients with chronic granulomatous disease despite the high prevalence of traditional risk factors raises questions about the role of NADPH oxidase in the pathogenesis of atherosclerosis. PMID: 25239440
• Four novel mutations in the NCF1, NCF2, and CYBB genes have been identified in chronic granulomatous disease patients in Morocco. PMID: 24596025
• This study suggests eupafolin attenuated COX-2 expression leading to reduced production of prostaglandin E2 by blocking the Nox2/p47(phox) pathway. PMID: 24967690
• p47(phox) and Rac2 accumulate only transiently at the phagosome at the onset of NADPH activity and detach from the phagosome before the end of reactive oxygen species production. PMID: 23870057
• There was an increase in p47-phox phosphorylation in neutrophils from myeloproliferative disorder patients with the JAK2 (V617F) mutation. PMID: 23975181
• Two novel mutations are identified in Greek patients with chronic granulomatous disease: one in NCF1 and one in cytochrome CYBB. PMID: 24081483
• Williams syndrome patients are at risk for increased aortic stiffness. This vascular stiffness is caused by elastin insufficiency and is modified by NCF1 copy number. PMID: 24126171
• Data show that curcumin-loaded polyvinylpyrrolidone nanoparticles (CURN) decreased the expression of ICAM-1, inhibited NADPH oxidase (NOX)-derived ROS generation, and reduced MAPKs and AP-1 transcription factor binding activities. PMID: 23671702
• This study identified a 10% incidence of diabetes in p47 (phox) deficient chronic granulomatous disease (CGD), but none in X-linked CGD. PMID: 23386289
• Three different cross-over points exist within the NCF1 gene cluster, indicating that autosomal p47(phox)-deficient CGD is genetically heterogeneous but can be dissected in detail by MLPA. PMID: 23688784
• Patients with p47(phox) hereditary deficiency have intermediate flow mediated dilation and oxidative stress compared to healthy subjects and patients with NOX2 deficiency. PMID: 23216310
• Defining p47-phox deficient Chronic Granulomatous Disease in a Malay family. PMID: 23393912
• Resveratrol decreases hyperglycemic induced superoxide production via up-regulation of SIRT1, induction of FOXO3a, and inhibition of p47phox in monocytes. PMID: 21813271
• There is no correlation between C923T(Ala308Val)polymorphism and cerebral hemorrhage in Han people in Hunan province. PMID: 21566280
• The low affinity and selectivity of the atypical phosphoinositide-binding site on the p47(phox) PX domain suggest that different types of phosphoinositides sequentially bind to the p47(phox) PX domain. PMID: 22493288
• Phosphorylation of p47(phox) at different serine sites plays distinct roles in endothelial cell response to TNFalpha stimulation. PMID: 22460559
• A diffuse cytosolic distribution of p47-phox was observed in neutrophils from HIV-infected patients. PMID: 22690528
• MLCK is essential for the translocation and association of cortactin and p47phox. PMID: 22219181
• An increased copy number of NCF1 may be protective against developing RA and supports previous findings of a role of NCF1 and the phagocyte NADPH oxidase complex in RA pathogenesis. PMID: 21728841
• Autosomal recessive mutational defects are the predominant subtype in Iranian patients with chronic granulomatous disease. PMID: 21789723
• Cooperation of p40(phox) with p47(phox) for Nox2-based NADPH oxidase activation during Fcgamma receptor (FcgammaR)-mediated phagocytosis. PMID: 21956105
• Data implicate p47phox as one of the sources of oxidative stress in diabetic islets or beta cells during hyperglycemia; evidence supports an accelerated Rac1-Nox-ROS-JNK1/2 signaling pathway leading to mitochondrial dysregulation. PMID: 21911753
• Protein disulfide isomerase redox-dependent association with p47(phox): evidence for an organizer role in leukocyte NADPH oxidase activation. PMID: 21791598
• Results demonstrate that PBEF can prime for PMN respiratory burst activity by promoting p40 and p47 translocation to the membrane. PMID: 21518975
• Direct contact of solid tumor cells and ECs activates endothelial NAD(P)H oxidase-mediated superoxide production. The oxidative stress contributes to EC apoptosis, which in turn facilitates tumor cell extravasation. PMID: 21506107
• A differential and agonist-dependent role of the p47(phox) PX domain for neutrophil NADPH oxidase activation. PMID: 20817944
• Granulomatous disease in Iran is predominantly due to mutations in p47-phox, while the number of mutations in p22-phox is roughly equal to that in gp91-phox, indicating that the genetics of CGD are ethnically variable. PMID: 20407811
• Loss of p47phox is associated with inflammasome activation resulting in chronic granulomatous disease. PMID: 20495074
• p47phox molecular activation for assembly of the neutrophil NADPH oxidase complex. PMID: 20592030
• Mutations in CYBB, NCF1, CYBA, or NCF2 may play a role in chronic granulomatous disease. PMID: 18546332
• There is an increased expression of NADPH oxidase p47(-PHOX) and p67(-PHOX) factor in idiopathic pulmonary fibrosis patients. PMID: 17651608
• All mutations and some polymorphisms identified in the NCF1 gene in the autosomal forms of chronic granulomatous disease are listed. Review. PMID: 20167518
• Expression of the p47phox subunit and NOX activity was evaluated in affected (superior and middle temporal gyri) and unaffected (cerebellum) brain regions from a longitudinally followed group of patients with varying degrees of cognitive impairment. PMID: 19929442

HGNC: 7660

OMIM: 233700

KEGG: hsa:653361

STRING: 9606.ENSP00000289473

UniGene: Hs.647047