Phospho-SF1 (Ser82) Antibody
Description
Introduction to Phospho-SF1 (Ser82) Antibody
Phospho-SF1 (Ser82) antibody is a rabbit polyclonal antibody targeting the phosphorylated form of SF1 at Ser82. SF1 (UniProt ID: Q15637) facilitates spliceosome assembly by binding to the branch point sequence (BPS) of pre-mRNA and regulates transcription. Phosphorylation at Ser82 modulates SF1’s interaction with U2AF2, affecting spliceosome dynamics .
Antibody Characteristics
Immunogen and Specificity
The antibody was generated using a synthesized non-phosphopeptide derived from human SF1 around Ser82. Key features include:
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Epitope: Phosphorylated Ser82 within the sequence S-P-SP-P-E .
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Cross-Reactivity: Validated in human tissues (e.g., lung cancer samples ) and mouse/rat models. Predictions extend to pig, zebrafish, and bovine .
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Validation: Specificity confirmed via blocking peptides (e.g., AF7395-BP), which eliminate signal in WB when pre-incubated with the target peptide .
Role in Spliceosome Regulation
Phosphorylation at Ser82 disrupts SF1’s interaction with U2AF2, impairing spliceosome assembly. This modification is critical for:
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Transcriptional Repression: SF1 binds EWSR1, FUS, and TAF15 to regulate RNA processing .
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Cellular Localization: Phosphorylated SF1 is predominantly nuclear .
Disease Relevance
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Cancer: Detected in lung cancer tissues via IHC, suggesting a role in oncogenic splicing alterations .
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Neurological Disorders: Interactions with RNA-binding proteins (e.g., RBM17) implicate SF1 in neurodegenerative diseases .
Validation and Quality Control
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Blocking Peptides: Available for competitive assays (e.g., AF7395-BP) .
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Phospho-Specificity: Does not recognize non-phosphorylated SF1 or SF1 phosphorylated at other residues .
Limitations and Restrictions
Product Specs
Target Background
- SF1 phosphorylation at Serine 20 enhances its binding affinity to U2AF(65) and reduces its binding to 3'-splice-site RNA. PMID: 28002734
- SF1 has a novel function in the initial recruitment of the U2 snRNP through direct interactions with two U2 snRNP-associated proteins. PMID: 26420826
- Post-translational modification of SF1, specifically phosphorylation of Serine 20, downregulates nuclear import of SF1 by altering the kinetic interaction between SF1's nuclear localization signal (NLS) and NLS receptor isoforms. PMID: 25043850
- PRPF40B directly interacts with SF1 and associates with U2AF(65. PMID: 25605964
- Gomafu indirectly regulates the function of the splicing factors SF1 and Celf3 by sequestering these proteins into separate nuclear bodies. PMID: 25145264
- Phosphorylation of the conserved SPSP motif and the SF1/U2AF interface are essential in vivo. PMID: 23273425
- The Zinc finger motif-1 (ZFM1) plays a crucial role in stabilizing a contractile SMC phenotype under basal or mildly activating conditions. PMID: 22906951
- Crystals of the 'mystery' domain of SF1 belonged to space group C2 and have most probable solvent contents of 64, 52 or 39% with three, four or five molecules per asymmetric unit, respectively. PMID: 21505248
- SF1 silencing impacted alternative splicing of endogenous transcripts, revealing a previously unknown role for SF1 and branch site-like sequences in splice site selection. PMID: 21062807
- The conformational changes induced by the assembly of the SF1/U2AF(65)/RNA complex optimally position the pre-mRNA splice site for subsequent splicing stages. PMID: 21146534
- SF3a60, 66, and 120 are essential for pre-mRNA splicing, while SF1 is not. PMID: 15647371
- SF1 was essential for the induction of alternative mRNA splicing by the beta-catenin/TCF4 complex. PMID: 17383426
- Puf60-UHM binds to ULM sequences in the splicing factors SF1, U2AF65, and SF3b155. PMID: 18974054
- The KH-QUA2 region of SF1 defines an enlarged KH (hn RNP K) fold which is necessary and sufficient for intron branched point sequence (BPS) binding. PMID: 11691992
