Phospho-WASF1 (Y125) Antibody
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Description
Biological Context of WASF1 and Y125 Phosphorylation
WASF1 (also termed WAVE1) is a member of the SCAR/WAVE family that regulates actin polymerization via interactions with the Arp2/3 complex. Phosphorylation at Tyr125 modulates its function in:
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Actin Cytoskeleton Remodeling: WASF1 transmits signals from GTPases (e.g., Rac) to drive membrane ruffling and lamellipodia formation .
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Mitochondrial Dynamics: Truncating mutations in WASF1 disrupt mitochondrial fission/fusion balance, leading to elongated mitochondria in fibroblasts .
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Neurodevelopmental Pathways: De novo WASF1 mutations are linked to intellectual disability, seizures, and autism spectrum disorders .
Phosphorylation at Tyr125 is mediated by ABL1 kinase and reversed by PTPN12 phosphatase, influencing WASF1’s interaction with downstream effectors like SORBS2 .
3.1. Neurodevelopmental Disorders
A 2018 study identified de novo truncating WASF1 mutations (e.g., p.Arg506Ter) in five individuals with severe neurodevelopmental impairments :
| Feature | Observation |
|---|---|
| Clinical Phenotypes | Intellectual disability, absent speech, seizures, autistic traits |
| Cellular Impact | Reduced full-length WASF1 levels (~50% of controls) and truncated protein expression (~15–25%) |
| Mitochondrial Defects | Elongated mitochondrial structures in patient-derived fibroblasts |
3.2. Amyloid-β Regulation
WASF1 interacts with the APP intracellular domain (AICD) to suppress amyloid-β production. AICD overexpression reduces β-secretase activity, highlighting WASF1’s role in Alzheimer’s disease pathways .
3.3. Antibody Validation Data
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Western Blot: Detects a ~75 kDa band (full-length WASF1) and a truncated ~70 kDa form in mutant cells .
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IHC/IF: Strong staining in human colon carcinoma tissues, blocked by phospho-specific peptides .
Associated Pathways and Interactions
Product Specs
Buffer
The antibody is provided as a liquid solution in phosphate-buffered saline (PBS) containing 50% glycerol, 0.5% bovine serum albumin (BSA), and 0.02% sodium azide.
Form
Liquid
Lead Time
Typically, we can ship the products within 1-3 business days after receiving your order. The delivery time may vary depending on the shipping method and location. Please consult your local distributors for specific delivery times.
Synonyms
WASP family; verprolin homology domain-containing protein 1 antibody; WASP family member 1 antibody; FLJ31482 antibody; homology of dictyostelium scar 1 antibody; KIAA0269 antibody; Protein WAVE-1 antibody; Protein WAVE1 antibody; scar; Dictyostelium; homology of; 1 antibody; SCAR1 antibody; Similar to a plant extensin like protein antibody; Verprolin homology domain containing protein 1 antibody; Verprolin homology domain-containing protein 1 antibody; WAS protein family; member 1 antibody; Wasf1 antibody; WASF1_HUMAN antibody; WASL antibody; WASP family 1 antibody; WASP family protein member 1 antibody; WASP family; verprolin homology domain-containing protein antibody; WAVE antibody; WAVE1 antibody; Wiskott Aldrich syndrome protein family member 1 antibody; Wiskott-Aldrich syndrome protein family member 1 antibody
Target Names
Uniprot No.
Target Background
Function
WASF1 (WASP Family Verprolin Homologous 1) is a downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. It promotes the formation of actin filaments and is part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization through its interaction with the Arp2/3 complex. As a component of the WAVE1 complex, WASF1 is required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes. It is also involved in the regulation of mitochondrial dynamics.
Gene References Into Functions
- A study on gene expression variability markers in early-stage human embryos identified WASF1 as a putative expression variability marker for the 3-day, 8-cell embryo stage. PMID: 26288249
- Research suggests that WAVE1 is a crucial pro-autophagic protein capable of enhancing cell survival and regulating chemoresistance in leukemia cells potentially through the Beclin1/Bcl-2 and Beclin1/PI3K- complex-dependent pathways. PMID: 27035872
- Findings implicate a contributory role of WAVE1 and -3 to the metastatic phenotype of PC-3 cells through their interaction with the ARP2/3 complex. PMID: 26977009
- Research proposes that WASF1 status defines a subtype of androgen deprivation therapy -resistant prostate cancer patients. PMID: 25906751
- Studies suggest a role for ARF6 in linking EGF-receptor signaling to Rac1 recruitment and activation at the plasma membrane to promote breast cancer cell directed migration. PMID: 25799492
- A decrease in amounts of WASF1 mRNA has been observed in human Alzheimer's disease brains, suggesting clinical relevance of the negative feedback circuit involved in homeostatic regulation of Abeta production. PMID: 26280122
- The D620N mutation in VPS35 restricts WASH complex recruitment to endosomes, revealing a novel role for the WASH complex in autophagosome formation. PMID: 24819384
- WAVE1 exhibits unique activities independent of the Arp2/3 complex that can govern both the growth rates and architectures of actin filament networks. Elongation inhibitory effects of WAVE1 were mapped to its WH2 ("V") domain. PMID: 25473116
- The WAVE complex is the primary activator of the Arp2/3 complex for actin filament nucleation and assembly in the lamellipodia of moving cells. PMID: 25355952
- WAVE1 may promote the proliferative and invasive malignant behaviors through the activation of the PI3K/AKT and p38MAPK signaling pathways in epithelial ovarian cancer. PMID: 23680521
- The Scar/WAVE regulatory complex and N-WASP play opposing roles in 3D epithelial cell migration. PMID: 23273897
- mRNAs encoding structural and regulatory components of the WAVE complex are localized to the leading edge of the cell, suggesting that localized protein synthesis plays a pivotal role in controlling cell spreading and migration. PMID: 23452202
- Studies find that WAVE1 overexpression is associated with an unfavorable prognosis. WAVE1 is an independent prognostic factor for EOC, suggesting that it is a novel and crucial predictor for EOC metastasis. PMID: 22721732
- mDia1 and WAVE2 are important Src homology 3 domain partners of IRSp53 in forming filopodia. PMID: 22179776
- Arf GTPases may be central components in WAVE signaling, acting directly, alongside Rac1. PMID: 21844371
- WAVE1 might be involved in the migration and invasion of K562 cells through regulation of the expression level of MMP-2. PMID: 19731823
- Higher levels of WAVE1 in the bone marrow indicate an unfavorable prognosis in children with AML. PMID: 20426950
- Dock3 induces axonal outgrowth by stimulating membrane recruitment of the WAVE complex. PMID: 20368433
- WAVE1 regulates Bcl-2 localization and phosphorylation in leukemia cells. PMID: 19890377
- Regarding the mechanism of regulation of WAVE1-induced actin nucleation by Rac1 and Nck, it is proposed that Rac1 and Nck cause dissociation of the WAVE1 complex, which releases active WAVE1-HSPC300 and leads to actin nucleation. PMID: 12181570
- WAVE1 may act as a scaffold to recruit the NADPH oxidase to a complex involved with both cytoskeletal regulation and downstream JNK activation. PMID: 12855698
- The 3 WAVE isoforms exhibit common and distinct features and may potentially be involved in the regulation of the actin cytoskeleton in platelets. PMID: 15280206
- Dictyostelium discoideum has been used to remove genetically SCAR complex members to ascertain their specific roles. PMID: 15506982
- WAVE-1 expression was associated with megakaryocytic differentiation; WAVE-1 and WAVE-2 moved from a detergent-soluble cytosolic fraction to insoluble cytoskeleton fraction after platelet aggregation. PMID: 15670045
- NESH (Abi-3), like Abi-1 and Abi-2, is a component of the Abi/WAVE complex, but likely plays a different role in the regulation of c-Abl. PMID: 17101133
- The Hem-1/Nap1 component of the Scar/WAVE complex localizes to propagating waves that appear to organize the leading edge of a motile neutrophil. PMID: 17696648
- WAVE1 is critical for the formation of oligodendrocyte lamellae and myelin sheaths--REVIEW. PMID: 17901257
- WAVE1 is involved in multi-drug resistance through regulation of the level of mdrl and Bcl-2. PMID: 17939402
- WAVE1 dephosphorylation and activation are likely associated with mitochondrial redistribution and dendritic spine morphogenesis. PMID: 18287015
- Results suggest that WAVE and the Arp2/3 complex jointly orchestrate different types of actin-based plasma membrane protrusions by promoting ruffling and inhibiting mDia2-induced filopodia. PMID: 18516090
- The WAVE1 expression increased in children with ALL. WAVE1 may be related to the development of ALL and may serve as a marker for the evaluation of the severity of ALL in children. PMID: 18947485
- WAVE1 and p22phox expression in PBMCs increased and was associated with the disease course in children with acute lymphocytic leukemia (ALL). PMID: 19222940
- WAVE accumulation may be involved in Abeta/amyloid precursor protein mediated-tangle modification. PMID: 19497998
Database Links
Protein Families
SCAR/WAVE family
Subcellular Location
Cytoplasm, cytoskeleton. Cell junction, synapse. Cell junction, focal adhesion.
Tissue Specificity
Highly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small intestine and peripheral blood.
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